Glutamine synthetase antibody [GT1055]

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:500.

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:250.
Red: PKC alpha protein stained by PKC alpha antibody [GT1876] (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytoplasm by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:250.
Red: GFAP, stained by GFAP antibody (GTX27260) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Glutamine synthetase stained by Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:5000.

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX630654) diluted at 1:1000.

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:500. Blue: DAPI

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:1000

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:5000.