APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
U87-MG , SK-N-SH , IMR32 , SK-N-AS , 293T , HeLa , HepG2 , mouse eye , mouse brain , rat brain , DDDDK-tagged GLUL-transfected 293T
Predict Reactivity
Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human Glutamine synthetase Recombinant protein.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888230
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59
Cellular Localization
Cytoplasm
Background
Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX630654 ICC/IF Image
Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytoplasm by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:250. Red: GFAP, stained by GFAP antibody (GTX27260) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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GTX630654 IHC-Fr Image
Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:250. Red: PKC alpha protein stained by PKC alpha antibody [GT1876] (GTX130453) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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GTX630654 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:500.
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GTX630654 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:5000.
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GTX630654 WB Image
Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg HeLa whole cell lysate/extract C. 30 μg HepG2 whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:1000
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GTX630654 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX630654 IHC-Fr Image
Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:500. Blue: DAPI
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GTX630654 WB Image
Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by western blot analysis. A. 30 μg U87-MG whole cell lysate/extract B. 30 μg SK-N-SH whole cell lysate/extract C. 30 μg IMR32 whole cell lysate/extract D. 30 μg SK-N-AS whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:1000
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GTX630654 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX630654) diluted at 1:1000.
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GTX630654 IHC-P Image
Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX630654 IHC-P Image
Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatoma. Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX630654 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:5000.
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GTX630654 IHC-P Image
Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
REVIEW
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