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Glutamine synthetase antibody [GT1055]

KO/KD Overexpression
GTX630654_41603_20160824_IHC-Fr_18121411_202.jpg
GTX630654_41603_20160630_WB_shRNA_watermark_18121411_962.jpg
GTX630654_41603_20161004_IFA_18121411_293.jpg
GTX630654_41603_20151002_IHC-Fr_M_18121411_197.jpg
GTX630654_41603_IHC_18121411_848.jpg
GTX630654_41603_20180928_WB_B_18121411_558.jpg
GTX630654_41603_20160825_WB_M_eye_18121411_922.jpg
GTX630654_41603_WB_18121411_737.jpg
GTX630654_41603_IHC_M_18121411_381.jpg
GTX630654_41603_20160901_WB_M_brain_18121411_765.jpg
GTX630654_41603_20160901_WB_R_brain_18121411_124.jpg
GTX630654_41603_IHC_R_18121411_752.jpg
GTX630654_41603_WB_2_18121411_169.jpg

Cat No. GTX630654

Host Mouse
Clonality Monoclonal
Clone Name GT1055
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , mouse eye , rat brain

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Glutamine synthetase Recombinant protein.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GLNS Antibody, GS Antibody, PIG59 Antibody, PIG43 Antibody, glutamate-ammonia ligase Antibody, GLUL Antibody

Cellular Localization

Cytoplasm

Background

Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX630654_41603_20160824_IHC-Fr_18121411_202.jpg

GTX630654 IHC-Fr Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:250.
Red: PKC alpha protein stained by PKC alpha antibody [GT1876] (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX630654_41603_20160630_WB_shRNA_watermark_18121411_962.jpg

GTX630654 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:500.

GTX630654_41603_20161004_IFA_18121411_293.jpg

GTX630654 ICC/IF Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytoplasm by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:250.
Red: GFAP, stained by GFAP antibody (GTX27260) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX630654_41603_20151002_IHC-Fr_M_18121411_197.jpg

GTX630654 IHC-Fr Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:500. Blue: DAPI

GTX630654_41603_IHC_18121411_848.jpg

GTX630654 IHC-P Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX630654_41603_20180928_WB_B_18121411_558.jpg

GTX630654 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX630654_41603_20160825_WB_M_eye_18121411_922.jpg

GTX630654 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX630654) diluted at 1:1000.

GTX630654_41603_WB_18121411_737.jpg

GTX630654 WB Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:1000

GTX630654_41603_IHC_M_18121411_381.jpg

GTX630654 IHC-P Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX630654_41603_20160901_WB_M_brain_18121411_765.jpg

GTX630654 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:5000.

GTX630654_41603_20160901_WB_R_brain_18121411_124.jpg

GTX630654 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:5000.

GTX630654_41603_IHC_R_18121411_752.jpg

GTX630654 IHC-P Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX630654_41603_WB_2_18121411_169.jpg

GTX630654 WB Image

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
10 % SDS-PAGE
Glutamine synthetase antibody [GT1055] (GTX630654) dilution: 1:1000

REFERENCE
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169