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Glutamine synthetase antibody [GT7711]

Anti-Glutamine synthetase antibody [GT7711] used in IHC (Frozen sections) (IHC-Fr). GTX630657
Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657
Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657
Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657
Anti-Glutamine synthetase antibody [GT7711] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630657
Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657
Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657
Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657
Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657
Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657
Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657

Cat. No. GTX630657

Host

Mouse

Clonality

Monoclonal

Clone Name

GT7711

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse eye , 293T , DDDDK-tagged GLUL-transfected 293T

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Glutamine synthetase Recombinant protein.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888231

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59

Cellular Localization

Cytoplasm

Background

Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Glutamine synthetase antibody [GT7711] used in IHC (Frozen sections) (IHC-Fr). GTX630657

GTX630657 IHC-Fr Image

Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:250.
Red: PKC alpha protein stained by PKC alpha antibody [GT1876] (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657

GTX630657 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:500.

Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657

GTX630657 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GTX630657) diluted at 1:1000.

Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657

GTX630657 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:250000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Glutamine synthetase antibody [GT7711] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630657

GTX630657 ICC/IF Image

Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein in glial cells by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron & glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:500.
Red: Tau, a Tau marker, stained by Tau antibody (GTX130462) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657

GTX630657 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Glutamine synthetase antibody [GT7711] used in Western Blot (WB). GTX630657

GTX630657 WB Image

Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
Glutamine synthetase antibody [GT7711] (GTX630657) dilution: 1:1000

Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657

GTX630657 IHC-P Image

Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657

GTX630657 IHC-P Image

Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657

GTX630657 IHC-P Image

Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutamine synthetase antibody [GT7711] used in IHC (Paraffin sections) (IHC-P). GTX630657

GTX630657 IHC-P Image

Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Glutamine synthetase stained by Glutamine synthetase antibody [GT7711] (GTX630657) diluted at 1:200.
Red: beta Tubulin 3/ Tuj1 , a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations