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Glutamine synthetase antibody [HL2283]

Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336
Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336
Anti-Glutamine synthetase antibody [HL2283] used in IHC (Paraffin sections) (IHC-P). GTX638336
Anti-Glutamine synthetase antibody [HL2283] used in IHC (Paraffin sections) (IHC-P). GTX638336
Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336
Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336
Anti-Glutamine synthetase antibody [HL2283] used in IHC (Paraffin sections) (IHC-P). GTX638336
Anti-Glutamine synthetase antibody [HL2283] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638336
Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336

Cat. No. GTX638336

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2283

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length of human Glutamine synthetase.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59

Cellular Localization

Cytoplasm, cytosol,Microsome,Mitochondrion,Cell membrane

Background

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336

GTX638336 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336

GTX638336 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutamine synthetase antibody [HL2283] used in IHC (Paraffin sections) (IHC-P). GTX638336

GTX638336 IHC-P Image

Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutamine synthetase antibody [HL2283] used in IHC (Paraffin sections) (IHC-P). GTX638336

GTX638336 IHC-P Image

Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336

GTX638336 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336

GTX638336 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutamine synthetase antibody [HL2283] used in IHC (Paraffin sections) (IHC-P). GTX638336

GTX638336 IHC-P Image

Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat cerebellum.
Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutamine synthetase antibody [HL2283] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638336

GTX638336 ICC/IF Image

Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein at glia by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:250.
Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Glutamine synthetase antibody [HL2283] used in Western Blot (WB). GTX638336

GTX638336 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations