GeneTex
United States (US)

Glutamine synthetase antibody

KO/KD
GTX32627_20180530_IHC-P_2_18121410_459.jpg
GTX32627_20180530_ICCIF_18121410_929.jpg
GTX32627_20180530_IHC-P_18121410_507.jpg
GTX32627_20180530_IHC-P_1_18121410_158.jpg
GTX32627_20180822_WB_18121410_570.jpg
GTX32627_20180530_WB_18121410_228.jpg

Cat No. GTX32627

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

42 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-373 of human GLUL (NP_001028228.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PIG43 Antibody , PIG59 Antibody , GLUL Antibody , GLNS Antibody , GS Antibody

Background

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
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Database

Research Area

DATA IMAGES
GTX32627_20180530_IHC-P_2_18121410_459.jpg

GTX32627 IHC-P Image

IHC-P analysis of mouse stomach tissue using GTX32627 Glutamine synthetase antibody.
Dilution : 1:100

GTX32627_20180530_ICCIF_18121410_929.jpg

GTX32627 ICC/IF Image

ICC/IF analysis of MCF-7 cell using GTX32627 Glutamine synthetase antibody.
Blue : DAPI

GTX32627_20180530_IHC-P_18121410_507.jpg

GTX32627 IHC-P Image

IHC-P analysis of human stomach tissue using GTX32627 Glutamine synthetase antibody.
Dilution : 1:100

GTX32627_20180530_IHC-P_1_18121410_158.jpg

GTX32627 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX32627 Glutamine synthetase antibody.
Dilution : 1:100

GTX32627_20180822_WB_18121410_570.jpg

GTX32627 WB Image

KO/KD

WB analysis of wild type and Glutamine synthetase knockout cell lysate using GTX32627 Glutamine synthetase antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32627_20180530_WB_18121410_228.jpg

GTX32627 WB Image

WB analysis of various samples using GTX32627 Glutamine synthetase antibody.
Dilution : 1:1000
Loading : 25μg per lane