APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-373 of human GLUL (NP_001028228.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59
Cellular Localization
Cytoplasm, cytosol,Microsome,Mitochondrion,Cell membrane
Background
The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Database
Research Area
DATA IMAGES
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GTX32627 IHC-P Image
IHC-P analysis of human stomach tissue using GTX32627 Glutamine synthetase antibody.
Dilution : 1:100
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GTX32627 IHC-P Image
IHC-P analysis of mouse stomach tissue using GTX32627 Glutamine synthetase antibody.
Dilution : 1:100
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GTX32627 ICC/IF Image
ICC/IF analysis of MCF7 cells using GTX32627 Glutamine synthetase antibody.
Blue : DAPI
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GTX32627 IHC-P Image
IHC-P analysis of mouse lung tissue using GTX32627 Glutamine synthetase antibody.
Dilution : 1:100
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GTX32627 WB Image
WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX32627 Glutamine synthetase antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX32627 WB Image
WB analysis of various sample lysates using GTX32627 Glutamine synthetase antibody.
Dilution : 1:1000
Loading : 25μg per lane
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REFERENCE
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REVIEW
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