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Glutamyl hydrolase gamma antibody [N1C3]

Anti-Glutamyl hydrolase gamma antibody [N1C3] used in Western Blot (WB). GTX109123
Anti-Glutamyl hydrolase gamma antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109123

Cat. No. GTX109123

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Glutamyl hydrolase gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Glutamyl hydrolase gamma Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950378

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gamma-glutamyl hydrolase , GH

Cellular Localization

Secreted , extracellular space , Lysosome , Melanosome

Background

This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Glutamyl hydrolase gamma antibody [N1C3] used in Western Blot (WB). GTX109123

GTX109123 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX109123 diluted at 1:1000

Anti-Glutamyl hydrolase gamma antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109123

GTX109123 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft , using Glutamyl hydrolase gamma (GTX109123) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Kuo WT et al. Oncol Rep 2017; (Epub) High * sensitivity of docetaxel-resistant A549 cells is mediated by TP53 status and downregulated thymidylate synthase.
Application : WB
Chiu LY et al. Oncogene 2016; (Epub) The ERK-ZEB1 pathway mediates epithelial-mesenchymal transition in * resistant lung cancer cells with suppression by vinca alkaloids.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169