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Glutathione Reductase antibody [N2C2], Internal

Anti-Glutathione Reductase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

Cat No. GTX114199

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

HCT116 , mouse eye , mouse brain , PC-12 , Rat-2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glutathione Reductase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620787

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glutathione-disulfide reductase , GR , HEL-75 , HEL-S-122m

Cellular Localization

Mitochondrion , Cytoplasm

Background

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Glutathione Reductase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114199

GTX114199 IHC-P Image

Glutathione reductase antibody [N2C2], Internal detects Glutathione reductase protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Glutathione reductase antibody [N2C2], Internal (GTX114199) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione reductase (GTX114199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione reductase (GTX114199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutathione Reductase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114199

GTX114199 IHC-P Image

Glutathione reductase antibody [N2C2], Internal detects Glutathione reductase protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Glutathione reductase antibody [N2C2], Internal (GTX114199) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione Reductase antibody (GTX114199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione Reductase antibody [N2C2], Internal (GTX114199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Glutathione Reductase antibody [N2C2], Internal Cat No. GTX114199

Rating ( Average 5 based on 4 users reviews)
Application
Western Blot(WB) ( Average 5 based on 4 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Proteome Sci in 2014.PMID: 24405781
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oxid Med Cell Longev in 2019.PMID: 31534619
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oxid Med Cell Longev in 2019.PMID: 31534619
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oxid Med Cell Longev in 2019.PMID: 31534619
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf