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Glutathione Reductase antibody [N2C2], Internal

KO/KD Orthogonal
Anti-Glutathione Reductase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199
Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

Cat. No. GTX114199

Reference 14

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, EM

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Glutathione Reductase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
EM Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

PC-12 , Rat-2 , mouse brain , mouse eye

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glutathione Reductase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620787

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione-disulfide reductase , GR , HEL-75 , HEL-S-122m

Cellular Localization

Mitochondrion , Cytoplasm

Background

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Glutathione Reductase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114199

GTX114199 IHC-P Image

Glutathione reductase antibody [N2C2], Internal detects Glutathione reductase protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Glutathione reductase antibody [N2C2], Internal (GTX114199) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione Reductase antibody [N2C2], Internal (GTX114199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutathione Reductase antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114199

GTX114199 IHC-P Image

Glutathione reductase antibody [N2C2], Internal detects Glutathione reductase protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Glutathione reductase antibody [N2C2], Internal (GTX114199) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione Reductase antibody (GTX114199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione reductase (GTX114199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutathione reductase (GTX114199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

KO/KD

The data was published in the journal Proteome Sci in 2014. PMID: 24405781

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31534619

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31534619

Anti-Glutathione Reductase antibody [N2C2], Internal used in Western Blot (WB). GTX114199

GTX114199 WB Image

Orthogonal

The data was published in the journal Oxid Med Cell Longev in 2019. PMID: 31534619

REFERENCE

Application Reference

M Brzozowa-Zasada et al. Int J Mol Sci 2024; 25 (2) : 1097 Glutathione Reductase Expression and Its Prognostic Significance in Colon Cancer
Application : WB, IHC-P, EM
KY Lee et al. Ecotoxicol Environ Saf 2023; Downregulation of TAZ elicits a mitochondrial redox imbalance and ferroptosis in lung epithelial cells exposed to diesel exhaust particles
Application : WB
Pek E et al. J Turk Ger Gynecol Assoc 2020; 21 (4) : 243-254 The immunohistochemical and histologic effects of contrast medium on uterus, fallopian tubes and ovaries, given during hysterosalpingography: rat study
Ramos H et al. Antioxidants (Basel) 2020; 9 (9)
Application : WB
Reactivity : Mouse
Bai X et al. Oxid Med Cell Longev 2019; 2019 : 2432416 Sesamin Enhances Nrf2-Mediated Protective Defense against Oxidative Stress and Inflammation in Colitis via AKT and ERK Activation.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
M Brzozowa-Zasada et al. Int J Mol Sci 2024; 25 (2):1097 Glutathione Reductase Expression and Its Prognostic Significance in Colon Cancer
Application : WB, IHC-P, EM
KY Lee et al. Ecotoxicol Environ Saf 2023; Downregulation of TAZ elicits a mitochondrial redox imbalance and ferroptosis in lung epithelial cells exposed to diesel exhaust particles
Application : WB
Pek E et al. J Turk Ger Gynecol Assoc 2020; 21 (4):243-254 The immunohistochemical and histologic effects of contrast medium on uterus, fallopian tubes and ovaries, given during hysterosalpingography: rat study
Ramos H et al. Antioxidants (Basel) 2020; 9 (9)
Application : WB
Reactivity : Mouse
Bai X et al. Oxid Med Cell Longev 2019; 2019:2432416 Sesamin Enhances Nrf2-Mediated Protective Defense against Oxidative Stress and Inflammation in Colitis via AKT and ERK Activation.
Application : WB
Reactivity : Human
Jeongsu Han et al. Oxidative Medicine and Cellular Longevity 2019; 2019:Article ID 2432416 Sesamin Enhances Nrf2-Mediated Protective Defense against Oxidative Stress and Inflammation in Colitis via AKT and ERK Activation.
Application : WB
Reactivity : Human
Saudrais E et al. Am J Physiol Cell Physiol 2018; (Epub) Choroid plexus glutathione peroxidases are instrumental in protecting the brain fluid environment from hydroperoxides during postnatal development.
Maclean KN et al. FASEB J 2017; (Epub) Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism.
Application : WB
Reactivity : Mouse
Zimnol A et al. Am J Physiol Renal Physiol 2017;:ajprenal.00183.2017 Angiotensin II type 1a receptor-deficient mice develop angiotensin II-induced oxidative stress and DNA damage without blood pressure increase.
Application : WB
Reactivity : Mouse
Wang YY et al. Head Neck 2017; (Epub) Synthetic β-nitrostyrene derivative CYT-Rx20 as inhibitor of oral cancer cell proliferation and tumor growth through glutathione suppression and reactive oxygen species induction.
Application : WB
Reactivity : Human
Huang CS et al. Biochem Pharmacol 2015; 93 (3):352-61 Shikonin inhibits oxidized LDL-induced monocyte adhesion by suppressing NFκB activation via up-regulation of PI3K/Akt/Nrf2-dependent antioxidation in EA.hy926 endothelial cells.
Application : WB
Reactivity : Human
Chi-Rei Wu et al. Chemico-Biological Interactions 2015; 225:40–46 Carnosic acid protects against 6-hydroxydopamine-induced neurotoxicity in in-vivo and in-vitro model of Parkinson’s disease: Involvement of antioxidative enzymes induction
Application : WB
Reactivity : Rat
Chou HC et al. Proteome Sci 2014; 12 (1):2 Effect of glutathione reductase knockdown in response to UVB-induced oxidative stress in human lung adenocarcinoma.
Application : WB
Reactivity : Human
Fan CY et al. Electrophoresis 2013; 34 (24):3305-14 Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169