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Glutathione S-transferase kappa 1 antibody

Anti-Glutathione S-transferase kappa 1 antibody used in Western Blot (WB). GTX117326
Anti-Glutathione S-transferase kappa 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117326
Anti-Glutathione S-transferase kappa 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117326
Anti-Glutathione S-transferase kappa 1 antibody used in Western Blot (WB). GTX117326

Cat. No. GTX117326

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A431 , HeLa

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glutathione S-transferase kappa 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173002

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione S-transferase kappa 1 , GST , GST 13-13 , GST13 , GST13-13 , GSTK1-1 , hGSTK1

Cellular Localization

Peroxisome

Background

This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES

Anti-Glutathione S-transferase kappa 1 antibody used in Western Blot (WB). GTX117326

GTX117326 WB Image

Wild-type (WT) and Glutathione S-transferase kappa 1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Glutathione S-transferase kappa 1 antibody (GTX117326) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glutathione S-transferase kappa 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117326

GTX117326 ICC/IF Image

Glutathione S-transferase kappa 1 antibody detects GSTK1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GSTK1 protein stained by Glutathione S-transferase kappa 1 antibody (GTX117326) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-Glutathione S-transferase kappa 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117326

GTX117326 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Glutathione S-transferase kappa 1(GTX117326) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Glutathione S-transferase kappa 1 antibody used in Western Blot (WB). GTX117326

GTX117326 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
12% SDS PAGE
GTX117326 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169