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Glycerol kinase antibody [N1N3-2]

Anti-Glycerol kinase antibody [N1N3-2] used in Western Blot (WB). GTX107474
Anti-Glycerol kinase antibody [N1N3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107474

Cat. No. GTX107474

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Glycerol kinase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Bovine, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glycerol kinase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950399

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycerol kinase , GK1 , GKD

Cellular Localization

Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm

Background

The product of this gene belongs to the FGGY kinase family of proteins and encodes glycerol kinase. Glycerol kinase is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Defects in this gene are the cause of glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Glycerol kinase antibody [N1N3-2] used in Western Blot (WB). GTX107474

GTX107474 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5 % SDS PAGE
GTX107474 diluted at 1:1000

Anti-Glycerol kinase antibody [N1N3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107474

GTX107474 ICC/IF Image

Glycerol kinase antibody [N1N3-2] detects Glycerol kinase protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: Glycerol kinase protein stained by Glycerol kinase antibody [N1N3-2] (GTX107474) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169