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Glycine Receptor alpha 1 antibody [N1C1]

Anti-Glycine Receptor alpha 1 antibody [N1C1] used in Western Blot (WB). GTX106112
Anti-Glycine Receptor alpha 1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106112
Anti-Glycine Receptor alpha 1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX106112

Cat No. GTX106112

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Raji

Predict Reactivity

Mouse, Zebrafish, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glycine Receptor alpha 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glycine Receptor Alpha 1,Hkpx1,Sthe,Glra1

Cellular Localization

Cell junction , synapse , postsynaptic cell membrane; Multi-pass membrane protein , Cell membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is a subunit of a pentameric inhibitory glycine receptor. The receptor mediates postsynaptic inhibition in the central nervous system. Defects in this gene are a cause of startle disease (STHE), also known as hereditary hyperekplexia or congenital stiff-person syndrome. Two transcript variants encoding different isoforms have been found for this gene.

Database

Research Area

DATA IMAGES
Anti-Glycine Receptor alpha 1 antibody [N1C1] used in Western Blot (WB). GTX106112

GTX106112 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX106112 diluted at 1:1500

Anti-Glycine Receptor alpha 1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106112

GTX106112 ICC/IF Image

Glycine Receptor alpha 1 antibody [N1C1] detects Glycine Receptor alpha 1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glycine Receptor alpha 1 stained by Glycine Receptor alpha 1 antibody [N1C1] (GTX106112) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Glycine Receptor alpha 1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX106112

GTX106112 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using Glycine Receptor alpha1(GTX106112) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169