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Glycine Receptor alpha 2 antibody

Anti-Glycine Receptor alpha 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04297
Anti-Glycine Receptor alpha 2 antibody used in Western Blot (WB). GTX04297
Anti-Glycine Receptor alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX04297

Cat. No. GTX04297

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Glycine Receptor alpha 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Dog, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human GLRA2(Accession P23416), corresponding to amino acid residues E18-S45.

Purification

Purified by affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycine receptor alpha 2 , GLR

Cellular Localization

Postsynaptic cell membrane , Cell junction, synapse , Cell membrane

Background

The glycine receptor consists of two subunits, alpha and beta, and acts as a pentamer. The protein encoded by this gene is an alpha subunit and can bind strychnine. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]

Database

Research Area

DATA IMAGES

Anti-Glycine Receptor alpha 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04297

GTX04297 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX04297 Glycine Receptor alpha 2 antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-Glycine Receptor alpha 2 antibody used in Western Blot (WB). GTX04297

GTX04297 WB Image

WB analysis of NIH-3T3 cell lysates using GTX04297 Glycine Receptor alpha 2 antibody. The lane on the left was treated with blocking peptide.

Anti-Glycine Receptor alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX04297

GTX04297 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX04297 Glycine Receptor alpha 2 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399