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Glycine Receptor alpha 2 antibody

Anti-Glycine Receptor alpha 2 antibody used in Western Blot (WB). GTX105634
Anti-Glycine Receptor alpha 2 antibody used in Western Blot (WB). GTX105634
Anti-Glycine Receptor alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105634

Cat No. GTX105634

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

293T , A431 , HepG2 , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glycine Receptor alpha 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glycine receptor alpha 2 , GLR

Cellular Localization

Cell junction , synapse , postsynaptic cell membrane; Multi-pass membrane protein , Cell membrane; Multi-pass membrane protein

Background

The glycine receptor consists of two subunits, alpha and beta, and acts as a pentamer. The protein encoded by this gene is an alpha subunit and can bind strychnine. Several transcript variants encoding different isoforms have been found for this gene.

Database

Research Area

DATA IMAGES
Anti-Glycine Receptor alpha 2 antibody used in Western Blot (WB). GTX105634

GTX105634 WB Image

Sample (30 µg of whole cell lysate)
A: 293T
B: A431
C: HepG2
10% SDS PAGE
GTX105634 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glycine Receptor alpha 2 antibody used in Western Blot (WB). GTX105634

GTX105634 WB Image

Glycine Receptor alpha 2 antibody detects GLRA2 protein by western blot analysis.
A. 50 µg mouse brain lysate/extract
10% SDS-PAGE
Glycine Receptor alpha 2 antibody (GTX105634) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glycine Receptor alpha 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX105634

GTX105634 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Glycine Receptor alpha 2(GTX105634) antibody at 1:750 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169