GeneTex
United States (US)

Glycogen synthase 1 antibody

GTX113453_40611_IHC_18121410_664.jpg
GTX113453_40611_WB_18121410_389.jpg
GTX113453_40611_WB_R_18121410_453.jpg

Cat No. GTX113453

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

A431 , PC-12

Predict Reactivity

Mouse, Zebrafish, Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glycogen synthase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GSY antibody, GYS antibody, GYS1 antibody, glycogen [starch] synthase, muscle antibody, glycogen synthase 1 (muscle) antibody

Background

Glycogen is a high molecular mass polysaccharide that serves as a repository of glucose for use in times of metabolic need. Glycogen synthase (EC 2.4.1.11) catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages (Pederson et al., 2004 [PubMed 15282316]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX113453_40611_IHC_18121410_664.jpg

GTX113453 IHC-P Image

Glycogen synthase 1 antibody detects GYS1 protein at cytosol on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
Glycogen synthase 1 antibody (GTX113453) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113453_40611_WB_18121410_389.jpg

GTX113453 WB Image

Glycogen synthase 1 antibody detects Glycogen synthase 1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with Glycogen synthase 1 antibody (GTX113453) diluted by 1:500

GTX113453_40611_WB_R_18121410_453.jpg

GTX113453 WB Image

Glycogen synthase 1 antibody detects Glycogen synthase 1 protein by western blot analysis. Rat tissue extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with Glycogen synthase 1 antibody (GTX113453) diluted by 1:500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169