GeneTex
United States (US)

Glycophorin A antibody [C1C3]

GTX100300_39456_IHC_19011506_378.jpg
GTX100300_39456_WB_19011506_610.jpg

Cat No. GTX100300

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

K562
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Glycophorin A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD235a antibody, GPA antibody, GPErik antibody, GPSAT antibody, GpMiIII antibody, HGpMiIII antibody, HGpMiV antibody, HGpMiX antibody, HGpMiXI antibody, HGpSta(C) antibody, MN antibody, MNS antibody, PAS-2 antibody, GYPA antibody, MN sialoglycoprotein antibody, "glycophorin A, GPA antibody", glycophorin SAT antibody, Mi.V glycoprotein (24 AA) antibody, glycophorin Sta type C antibody, glycophorin A (MN blood group) antibody, sialoglycoprotein alpha antibody, glycophorin MiV antibody, glycophorin MiI antibody, glycophorin MiX antibody, glycophorin MiIII antibody, recombinant glycophorin A-B Miltenberger-DR antibody, glycophorin-A antibody, erythroid-lineage-specific membrane sialoglycoprotein antibody, glycophorin Erik antibody, glycophorin A (MNS blood group) antibody

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100300_39456_IHC_19011506_378.jpg

GTX100300 IHC-P Image

Glycophorin A antibody [C1C3] detects Glycophorin A protein at cytosol on human lung carcinoma by immunohistochemical analysis.
Sample: Glycophorin A antibody [C1C3] (GTX100300) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100300_39456_WB_19011506_610.jpg

GTX100300 WB Image

Sample (30 ug of whole cell lysate)
A: K562
15% SDS PAGE
GTX100300 diluted at 1:1000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169