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Glycophorin A antibody [C1C3]

Anti-Glycophorin A antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100300
Anti-Glycophorin A antibody [C1C3] used in Western Blot (WB). GTX100300

Cat. No. GTX100300

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Glycophorin A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

K562

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human Glycophorin A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240584

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycophorin A (MNS blood group) , CD235a , GPA , GPErik , GPSAT , HGpMiV , HGpMiXI , HGpSta(C) , MN , MNS , PAS-2

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Glycophorin A antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100300

GTX100300 IHC-P Image

Glycophorin A antibody [C1C3] detects Glycophorin A protein at cytosol on human lung carcinoma by immunohistochemical analysis.
Sample: Glycophorin A antibody [C1C3] (GTX100300) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Glycophorin A antibody [C1C3] used in Western Blot (WB). GTX100300

GTX100300 WB Image

Sample (30 ug of whole cell lysate)
A: K562
15% SDS PAGE
GTX100300 diluted at 1:1000

REFERENCE

Application Reference

Fernando MR et al. PLoS One 2017; 12 (8) : e0183915 New evidence that a large proportion of human blood plasma cell-free DNA is localized in exosomes.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169