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Glycophorin A antibody [HIR2] (FITC)

Cat No. GTX80122

Host Mouse
Clonality Monoclonal
Clone Name HIR2
Isotype IgG2b
Application IHC-Fr, FACS, AGG
Reactivity Human
Package
100 test ($319)
APPLICATION

Application Note

Recommended Starting Dilutions:
FACS: This HIR2 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes and cell agglutination assay and can be used at approximately 0.1 μg per million cells.
Agglutination: The antibody HIR2 agglutinates untreated RBCs but failes to agglutinate papain-treated cells
Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

16 kDa. ( Note )

Specificity/Sensitivity

The antibody HIR2 recognizes N-terminal portion of glycophorin A (and weakly of glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.
PROPERTIES

Form

Liquid

Buffer

The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Antigen Species

Human

Immunogen

Synthetic peptide (Human, N-terminal)

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glycophorin A (Mns Blood Group),Cd235A,Gpa,Gperik,Gpsat,Hgpmiv,Hgpmixi,Hgpsta(C),Mn,Mns,Pas-2,Gypa

Cellular Localization

Appears to be colocalized with SLC4A1.

Background

CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin. Entrez Gene: 4q28.2-q31.1

Database

Research Area

REFERENCE
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Package List Price ($)
$ 319