Recommended Starting Dilutions:
FACS: This HIR2 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes and cell agglutination assay and can be used at approximately 0.1 μg per million cells.
Agglutination: The antibody HIR2 agglutinates untreated RBCs but failes to agglutinate papain-treated cells
Optimal dilutions should be determined experimentally by the researcher.
The antibody HIR2 recognizes N-terminal portion of glycophorin A (and weakly of glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.
The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Synthetic peptide (Human, N-terminal)
Fluorescein isothiocyanate (FITC)
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Glycophorin A (Mns Blood Group),Cd235A,Gpa,Gperik,Gpsat,Hgpmiv,Hgpmixi,Hgpsta(C),Mn,Mns,Pas-2,Gypa
Appears to be colocalized with SLC4A1.
CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin. Entrez Gene: 4q28.2-q31.1