APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , HeLa , HepG2 , A375
Predict Reactivity
Mouse, Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Glyoxalase I. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888066
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glyoxalase I , GLOD1 , GLYI , HEL-S-74
Background
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Glyoxalase I antibody [GT266] used in Western Blot (WB). GTX628889](/templates/default/js/lightbox/images/loading.gif "Anti-Glyoxalase I antibody [GT266] used in Western Blot (WB). GTX628889")
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GTX628889 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [GT266] (GTX628889) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628889 ICC/IF Image
Glyoxalase I antibody [GT266] detects Glyoxalase I protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glyoxalase I stained by Glyoxalase I antibody [GT266] (GTX628889) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
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GTX628889 IHC-P Image
Glyoxalase I antibody [GT266] detects Glyoxalase I protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.Glyoxalase I stained by Glyoxalase I antibody [GT266] (GTX628889) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX628889 WB Image
Glyoxalase I antibody [GT266] detects GLO1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
D. 30 μg A375 whole cell lysate/extract
12 % SDS-PAGE
Glyoxalase I antibody [GT266] (GTX628889) dilution: 1:1000
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REFERENCE
There are currently no references for Glyoxalase I antibody [GT266] (GTX628889). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Glyoxalase I antibody [GT266] (GTX628889). Be the first to share your experience with this product.
![Anti-Glyoxalase I antibody [GT266] used in Western Blot (WB). GTX628889](/upload/website/prouct_img/small/GTX628889/GTX628889_41295_20200807_WB_shRNA_watermark_w_23061202_809.webp "Anti-Glyoxalase I antibody [GT266] used in Western Blot (WB). GTX628889")
![Anti-Glyoxalase I antibody [GT266] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628889](/upload/website/prouct_img/small/GTX628889/GTX628889_41295_20180810_ICC_IF_w_23061202_789.webp "Anti-Glyoxalase I antibody [GT266] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628889")
![Anti-Glyoxalase I antibody [GT266] used in IHC (Paraffin sections) (IHC-P). GTX628889](/upload/website/prouct_img/small/GTX628889/GTX628889_41295_20180622_IHC-P_w_23061202_696.webp "Anti-Glyoxalase I antibody [GT266] used in IHC (Paraffin sections) (IHC-P). GTX628889")
![Anti-Glyoxalase I antibody [GT266] used in Western Blot (WB). GTX628889](/upload/website/prouct_img/small/GTX628889/GTX628889_41295_WB_w_23061202_285.webp "Anti-Glyoxalase I antibody [GT266] used in Western Blot (WB). GTX628889")
![Anti-Glyoxalase I antibody [GT266] used in Western Blot (WB). GTX628889](/upload/website/prouct_img/normal/GTX628889/GTX628889_41295_20200807_WB_shRNA_watermark_w_23061202_809.webp "Anti-Glyoxalase I antibody [GT266] used in Western Blot (WB). GTX628889")
![Anti-Glyoxalase I antibody [GT266] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628889](/upload/website/prouct_img/normal/GTX628889/GTX628889_41295_20180810_ICC_IF_w_23061202_789.webp "Anti-Glyoxalase I antibody [GT266] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628889")
![Anti-Glyoxalase I antibody [GT266] used in IHC (Paraffin sections) (IHC-P). GTX628889](/upload/website/prouct_img/normal/GTX628889/GTX628889_41295_20180622_IHC-P_w_23061202_696.webp "Anti-Glyoxalase I antibody [GT266] used in IHC (Paraffin sections) (IHC-P). GTX628889")
![Anti-Glyoxalase I antibody [GT266] used in Western Blot (WB). GTX628889](/upload/website/prouct_img/normal/GTX628889/GTX628889_41295_WB_w_23061202_285.webp "Anti-Glyoxalase I antibody [GT266] used in Western Blot (WB). GTX628889")