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Glyoxalase I antibody [GT552]

KO/KD Orthogonal
Anti-Glyoxalase I antibody [GT552] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628890
Anti-Glyoxalase I antibody [GT552] used in IHC (Paraffin sections) (IHC-P). GTX628890
Anti-Glyoxalase I antibody [GT552] used in Western Blot (WB). GTX628890
Anti-Glyoxalase I antibody [GT552] used in Western Blot (WB). GTX628890
Anti-Glyoxalase I antibody [GT552] used in Western Blot (WB). GTX628890
Anti-Glyoxalase I antibody [GT552] used in Western Blot (WB). GTX628890
Anti-Glyoxalase I antibody [GT552] used in Western Blot (WB). GTX628890

Cat. No. GTX628890

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT552

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Glyoxalase I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

293T , HepG2 , rat brain

Predict Reactivity

Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glyoxalase I. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888067

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glyoxalase I , GLOD1 , GLYI , HEL-S-74

Background

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Glyoxalase I antibody [GT552] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628890

GTX628890 ICC/IF Image

Glyoxalase I antibody [GT552] detects Glyoxalase I protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glyoxalase I stained by Glyoxalase I antibody [GT552] (GTX628890) diluted at 1:100.
Blue: Hoechst 33342 staining.

Anti-Glyoxalase I antibody [GT552] used in IHC (Paraffin sections) (IHC-P). GTX628890

GTX628890 IHC-P Image

Glyoxalase I antibody [GT552] detects GLO1 protein at cytosol and nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Glyoxalase I antibody [GT552] (GTX628890) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Glyoxalase I antibody [GT552] used in Western Blot (WB). GTX628890

GTX628890 WB Image

Glyoxalase I antibody [GT542] detects GLO1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
Glyoxalase I antibody [GT542] (GTX628890) dilution: 1:1000

Anti-Glyoxalase I antibody [GT552] used in Western Blot (WB). GTX628890

GTX628890 WB Image

Glyoxalase I antibody [GT542] detects GLO1 protein by Western blot analysis.
A. 50 μg rat brain lysate/extract
12 % SDS-PAGE
Glyoxalase I antibody [GT542] (GTX628890) dilution: 1:1000

Anti-Glyoxalase I antibody [GT552] used in Western Blot (WB). GTX628890

GTX628890 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [GT552] (GTX628890) diluted at 1:500.

Anti-Glyoxalase I antibody [GT552] used in Western Blot (WB). GTX628890

GTX628890 WB Image

Orthogonal

The data was published in the journal Elife in 2019. PMID: 31453805

Anti-Glyoxalase I antibody [GT552] used in Western Blot (WB). GTX628890

GTX628890 WB Image

Orthogonal

The data was published in the journal Elife in 2019. PMID: 31453805

REFERENCE

Application Reference

Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Yagensky O et al. Elife 2019; 8 Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations