Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [GT552] (GTX628890) diluted at 1:500.

Glyoxalase I antibody [GT542] detects GLO1 protein by Western blot analysis.
A. 50 μg rat brain lysate/extract
12 % SDS-PAGE
Glyoxalase I antibody [GT542] (GTX628890) dilution: 1:1000

Glyoxalase I antibody [GT542] detects GLO1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
Glyoxalase I antibody [GT542] (GTX628890) dilution: 1:1000

Glyoxalase I antibody [GT552] detects GLO1 protein at cytosol and nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Glyoxalase I antibody [GT552] (GTX628890) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Glyoxalase I antibody [GT552] detects Glyoxalase I protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glyoxalase I stained by Glyoxalase I antibody [GT552] (GTX628890) diluted at 1:100.
Blue: Hoechst 33342 staining.

The data was published in the journal Elife in 2019. PMID: 31453805

The data was published in the journal Elife in 2019. PMID: 31453805