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Glyoxalase I antibody [Glo1a]

Anti-Glyoxalase I antibody [Glo1a] used in Western Blot (WB). GTX15747
Anti-Glyoxalase I antibody [Glo1a] used in Western Blot (WB). GTX15747
Anti-Glyoxalase I antibody [Glo1a] used in IHC (Paraffin sections) (IHC-P). GTX15747

Cat. No. GTX15747

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 Glo1a

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse, Rat, Primate
Package
100 μg ($399)
See all Glyoxalase I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:800
IHC-P 1:800
IHC Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human GLOI-GST fusion protein.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glyoxalase I , GLOD1 , GLYI , HEL-S-74

Background

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Glyoxalase I antibody [Glo1a] used in Western Blot (WB). GTX15747

GTX15747 WB Image

WB analysis of 25 ug of various whole cell lysates using GTX15747 Glyoxalase I antibody [Glo1a].
Dilution : 1:1000

Anti-Glyoxalase I antibody [Glo1a] used in Western Blot (WB). GTX15747

GTX15747 WB Image

WB analysis of whole cell extracts (30 μg lysate) of MCF7 (Lane 1), HeLa (Lane 2), MDA-MB-231 (Lane 3), HL-60 (Lane 4), MOLT-4 (Lane 5), LNCaP (Lane 6), T-47D (Lane 7), U-87 MG (Lane 8) and A549 (Lane 9) using GTX15747 Glyoxalase I antibody [Glo1a].
Dilution : 1:1000

Anti-Glyoxalase I antibody [Glo1a] used in IHC (Paraffin sections) (IHC-P). GTX15747

GTX15747 IHC-P Image

IHC-P analysis of human prostate and stomach carcinoma tissue using GTX15747 Glyoxalase I antibody [Glo1a].
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes
Dilution : 1:800

REFERENCE

Application Reference

Cinzia Antognelli et al. Cancers (Basel) 2021; 13 (12) Glyoxalase-1-Dependent Methylglyoxal Depletion Sustains PD-L1 Expression in Metastatic Prostate Cancer Cells: A Novel Mechanism in Cancer Immunosurveillance Escape and a Potential Novel Target to Overcome PD-L1 Blockade Resistance.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399