APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 , HeLa , HepG2 , Mouse brain
Predict Reactivity
Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Glyoxalase I. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
GLOD1 antibody, GLYI antibody, GLO1 antibody, aldoketomutase antibody, glyoxalase domain containing 1 antibody, lactoylglutathione lyase antibody, S-D-lactoylglutathione methylglyoxal lyase antibody, lactoyl glutathione lyase antibody, methylglyoxalase antibody, ketone-aldehyde mutase antibody, glx I antibody, glyoxalase I antibody
Background
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008]
Database
DATA IMAGES
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GTX105792 WB Image
Sample (50 µg of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105792 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105792 ICC/IF Image
Glyoxalase I antibody [N1C3] detects Glyoxalase I protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glyoxalase I protein stained by Glyoxalase I antibody [N1C3] (GTX105792) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX105792 WB Image
Glyoxalase I antibody detects Glyoxalase I protein by Western blot analysis.
A. 30 μg A431 whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
Glyoxalase I antibody (GTX105792) dilution: 1:1000
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GTX105792 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using GLO1(GTX105792) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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