GeneTex
United States (US)

Glyoxalase I antibody [N1C3]

GTX105792_39946_WB_M_brain_19011506_696.jpg
GTX105792_41577_20150525_IFA_19011506_735.jpg
GTX105792_41577_WB_19011506_427.jpg
GTX105792_IHC_19011506_771.jpg

Cat No. GTX105792

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , Mouse brain

Predict Reactivity

Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glyoxalase I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GLOD1 antibody, GLYI antibody, GLO1 antibody, aldoketomutase antibody, glyoxalase domain containing 1 antibody, lactoylglutathione lyase antibody, S-D-lactoylglutathione methylglyoxal lyase antibody, lactoyl glutathione lyase antibody, methylglyoxalase antibody, ketone-aldehyde mutase antibody, glx I antibody, glyoxalase I antibody

Background

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX105792_39946_WB_M_brain_19011506_696.jpg

GTX105792 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105792 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105792_41577_20150525_IFA_19011506_735.jpg

GTX105792 ICC/IF Image

Glyoxalase I antibody [N1C3] detects Glyoxalase I protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glyoxalase I protein stained by Glyoxalase I antibody [N1C3] (GTX105792) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX105792_41577_WB_19011506_427.jpg

GTX105792 WB Image

Glyoxalase I antibody detects Glyoxalase I protein by Western blot analysis.
A. 30 μg A431 whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
Glyoxalase I antibody (GTX105792) dilution: 1:1000

GTX105792_IHC_19011506_771.jpg

GTX105792 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using GLO1(GTX105792) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169