Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [N1C3] (GTX105792) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 μg of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105792 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [N1C3] (GTX105792) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Glyoxalase I antibody [N1C3] detects Glyoxalase I protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glyoxalase I protein stained by Glyoxalase I antibody [N1C3] (GTX105792) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Glyoxalase I antibody [N1C3] detects Glyoxalase I protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
Glyoxalase I stained by Glyoxalase I antibody [N1C3] (GTX105792) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min