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Glyoxalase I antibody [N1C3]

Anti-Glyoxalase I antibody [N1C3] used in Western Blot (WB). GTX105792
Anti-Glyoxalase I antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105792
Anti-Glyoxalase I antibody [N1C3] used in Western Blot (WB). GTX105792
Anti-Glyoxalase I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105792

Cat No. GTX105792

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all Glyoxalase I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , Mouse brain

Predict Reactivity

Rat, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glyoxalase I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glyoxalase I , GLOD1 , GLYI , HEL-S-74

Background

The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Glyoxalase I antibody [N1C3] used in Western Blot (WB). GTX105792

GTX105792 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX105792 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glyoxalase I antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105792

GTX105792 ICC/IF Image

Glyoxalase I antibody [N1C3] detects Glyoxalase I protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glyoxalase I protein stained by Glyoxalase I antibody [N1C3] (GTX105792) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Glyoxalase I antibody [N1C3] used in Western Blot (WB). GTX105792

GTX105792 WB Image

Glyoxalase I antibody detects Glyoxalase I protein by Western blot analysis.
A. 30 μg A431 whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
Glyoxalase I antibody (GTX105792) dilution: 1:1000

Anti-Glyoxalase I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX105792

GTX105792 IHC-P Image

Glyoxalase I antibody [N1C3] detects Glyoxalase I protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
Glyoxalase I stained by Glyoxalase I antibody [N1C3] (GTX105792) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Chen SM et al. PLoS One 2020; 15 (1) : e0227838 Effect of prednisolone on glyoxalase 1 in an inbred mouse model of aristolochic acid nephropathy using a proteomics method with fluorogenic derivatization-liquid chromatography-tandem mass spectrometry.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169