GeneTex
United States (US)

Glypican 1 antibody [N3C3]

KO/KD Comparison Orthogonal
GTX104557_42326_20160728_WB_Benzyl glucosinolate_Tunicamycin_19011720_882.jpg
GTX104557_42326_20171110_WB_competitor_watermark_19011506_566.jpg
GTX104557_42326_20160509_WB_Tunicamycin_19011506_188.jpg
GTX104557_42326_20160509_WB_PNGase F_19011720_364.jpg
GTX104557_42326_20160509_WB_Fraction_2_19011506_879.jpg
GTX104557_42326_20160509_WB_M_brain_19011506_661.jpg
GTX104557_42697_20161215_WB_19011506_798.jpg
GTX104557_42326_20160505_ELISA_19011506_529.jpg
GTX104557_42326_20150505_IHC-P_19011506_319.jpg
GTX104557_42326_20160429_IP_19011506_291.jpg
GTX104557_42697_20170524_IFA_19011506_534.jpg
GTX104557_42326_20160509_WB_Fraction_19011506_899.jpg
GTX104557_42326_20160407_FACS_19011506_910.jpg
GTX104557_42326_20160509_WB_shRNA_watermark_19011506_828.jpg

Cat No. GTX104557

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IP, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

A431 , K562 , MCF-7 , AsPC-1 , MDA-MB-231 , MDA-MB-231 (24 μg/ml Tunicamycin for 16hr) , U87-MG , U87-MG membrane extracts , U87-MG conditioned medium , Mouse brain , mouse fetal brain , A431 (500 units PNGase F for 4 hr) , A431 (24 μg/ml Tunicamycin treatment for 16hr) , A431 (4 mM Benzyl glucosinolate treatment for 32hr) , A431(24 μg/ml Tunicamycin treatment for 16hr and 4 mM Benzyl glucosinolate treatment for 32hr)

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glypican 1,Glypican,Gpc1

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104557_42326_20160728_WB_Benzyl glucosinolate_Tunicamycin_19011720_882.jpg

GTX104557 WB Image

Orthogonal

Untreated (–) and treated (+) A431 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104557_42326_20171110_WB_competitor_watermark_19011506_566.jpg

GTX104557 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500 and competitor's antibody (sc-365000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX104557_42326_20160509_WB_Tunicamycin_19011506_188.jpg

GTX104557 WB Image

Orthogonal

Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761, 26203194 and 21932778

GTX104557_42326_20160509_WB_PNGase F_19011720_364.jpg

GTX104557 WB Image

Orthogonal

Untreated (–) and treated (+) A431 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761, 26203194 and 21932778

GTX104557_42326_20160509_WB_Fraction_2_19011506_879.jpg

GTX104557 WB Image

Orthogonal

U87-MG whole cell extract and conditioned medium (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761, 26203194 and 21932778

GTX104557_42326_20160509_WB_M_brain_19011506_661.jpg

GTX104557 WB Image

Mouse tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104557_42697_20161215_WB_19011506_798.jpg

GTX104557 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500.

GTX104557_42326_20160505_ELISA_19011506_529.jpg

GTX104557 ELISA Image

An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 antibody (GTX104557) at concentration ranged from 7.5 to 240 ng/mL.

GTX104557_42326_20150505_IHC-P_19011506_319.jpg

GTX104557 IHC-P Image

Immunohistochemical microscopy analysis of paraffin-embedded human normal pancreas (left) or pancreatic adenocarcinoma (grade II) (right) tissues using Glypican-1 antibody [N3C3] (GTX104557) at a 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104557_42326_20160429_IP_19011506_291.jpg

GTX104557 IP Image

Immunoprecipitation of Glypican 1 protein from MCF-7 whole cell extracts using 5 μg of Glypican 1 antibody [N3C3] (GTX104557).
Western blot analysis was performed using Glypican 1 antibody [N3C3] (GTX104557).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX104557_42697_20170524_IFA_19011506_534.jpg

GTX104557 ICC/IF Image

Glypican 1 antibody [N3C3] detects Glypican 1 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min.
Green: Glypican 1 protein stained by Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX104557_42326_20160509_WB_Fraction_19011506_899.jpg

GTX104557 WB Image

Orthogonal

U87-MG whole cell and membrane extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761,26203194 and 21932778

GTX104557_42326_20160407_FACS_19011506_910.jpg

GTX104557 FACS Image

Glypican 1 antibody [N3C3] (GTX104557) detects Glypican 1 protein by flow cytometry analysis.
Sample: A431 cell.
Black: Unlabelled sample was used as a control.
Red: Glypican 1 antibody [N3C3] (GTX104557) dilution: 1:100.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

GTX104557_42326_20160509_WB_shRNA_watermark_19011506_828.jpg

GTX104557 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761, 26203194 and 21932778

REFERENCE
REVIEW

Glypican 1 antibody [N3C3] Cat No. GTX104557

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 05-May-2016
Score :
Application Tested : WB
Sample : MCF-7
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane1: Non-infected control. Lane2: Mock control. Lane3_GPC1-shRNA(10) lentivirus infection. Lane4: GPC1-shRNA(30) lentivirus infection.
Date : Anonymous submitted on 05-May-2016
Score :
Application Tested : WB
Sample : MCF-7
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane1: Non-infected control. Lane2: Mock control. Lane3_GPC1-shRNA(10) lentivirus infection. Lane4: GPC1-shRNA(30) lentivirus infection.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169