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Glypican 1 antibody [N3C3]

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in ELISA (ELISA). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Immunoprecipitation (IP). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Flow cytometry (FACS). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104557
Anti-Glypican 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX104557
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

Cat No. GTX104557

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IP, ELISA
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500
ELISA 1:1000-1:10000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

A431 , K562 , MCF-7 , AsPC-1 , MDA-MB-231 , MDA-MB-231 (24 μg/ml Tunicamycin for 16hr) , U87-MG , U87-MG membrane extracts , U87-MG conditioned medium , Mouse brain , mouse fetal brain , A431 (500 units PNGase F for 4 hr) , A431 (24 μg/ml Tunicamycin treatment for 16hr) , A431 (4 mM Benzyl glucosinolate treatment for 32hr) , A431(24 μg/ml Tunicamycin treatment for 16hr and 4 mM Benzyl glucosinolate treatment for 32hr)

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glypican 1 , glypican

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500 and competitor's antibody (sc-365000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

Untreated (–) and treated (+) MDA-MB-231 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761, 26203194 and 21932778

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761, 26203194 and 21932778

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

U87-MG whole cell extract and conditioned medium (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761, 26203194 and 21932778

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

Mouse tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500.

Anti-Glypican 1 antibody [N3C3] used in ELISA (ELISA). GTX104557

GTX104557 ELISA Image

An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 antibody (GTX104557) at concentration ranged from 7.5 to 240 ng/mL.

Anti-Glypican 1 antibody [N3C3] used in Immunoprecipitation (IP). GTX104557

GTX104557 IP Image

Immunoprecipitation of Glypican 1 protein from MCF-7 whole cell extracts using 5 μg of Glypican 1 antibody [N3C3] (GTX104557).
Western blot analysis was performed using Glypican 1 antibody [N3C3] (GTX104557).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

U87-MG whole cell and membrane extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761,26203194 and 21932778

Anti-Glypican 1 antibody [N3C3] used in Flow cytometry (FACS). GTX104557

GTX104557 FACS Image

Glypican 1 antibody [N3C3] (GTX104557) detects Glypican 1 protein by flow cytometry analysis.
Sample: A431 cell.
Black: Unlabelled sample was used as a control.
Red: Glypican 1 antibody [N3C3] (GTX104557) dilution: 1:100.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22351761, 26203194 and 21932778

Anti-Glypican 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104557

GTX104557 ICC/IF Image

Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: Glypican 1 stained by Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-Glypican 1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX104557

GTX104557 IHC-P Image

Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human normal pancreas (left) and pancreatic cancer (right).
Glypican 1 stained by Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glypican 1 antibody [N3C3] used in Western Blot (WB). GTX104557

GTX104557 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (GTX104557) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Glypican 1 antibody [N3C3] Cat No. GTX104557

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 05-May-2016
Score :
Application Tested : WB
Sample : MCF-7
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane1: Non-infected control. Lane2: Mock control. Lane3_GPC1-shRNA(10) lentivirus infection. Lane4: GPC1-shRNA(30) lentivirus infection.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169