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Glypican 1 antibody

Anti-Glypican 1 antibody used in Western Blot (WB). GTX132672
Anti-Glypican 1 antibody used in Western Blot (WB). GTX132672
Anti-Glypican 1 antibody used in Western Blot (WB). GTX132672
Anti-Glypican 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132672

Cat No. GTX132672

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

A431 , K562 , MCF-7 , AsPC-1 , A431 (24 μg/ml Tunicamycin treatment for 16 hr) , A431 (4 mM Benzyl glucosinolate treatment for 32 hr) , A431(24 μg/ml Tunicamycin treatment for 16 hr and 4 mM Benzyl glucosinolate treatment for 32 hr)

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glypican 1 , glypican

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Secreted glypican-1: Secreted , extracellular space

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Glypican 1 antibody used in Western Blot (WB). GTX132672

GTX132672 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody (GTX132672) diluted at 1:500 and competitor's antibody (sc-365000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Glypican 1 antibody used in Western Blot (WB). GTX132672

GTX132672 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody (GTX132672) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glypican 1 antibody used in Western Blot (WB). GTX132672

GTX132672 WB Image

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody (GTX132672) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glypican 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132672

GTX132672 IHC-P Image

Glypican 1 antibody detects Glypican 1 protein at cell membrane in human pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreas, normal (left) and cancer (right).
Glypican 1 antibody (GTX132672) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169