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Glypican 1 antibody

Anti-Glypican 1 antibody used in Western Blot (WB). GTX132672
Anti-Glypican 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132672
Anti-Glypican 1 antibody used in Western Blot (WB). GTX132672
Anti-Glypican 1 antibody used in Western Blot (WB). GTX132672

Cat No. GTX132672

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

A431 , K562 , MCF-7 , AsPC-1 , A431 (24 μg/ml Tunicamycin treatment for 16 hr) , A431 (4 mM Benzyl glucosinolate treatment for 32 hr) , A431(24 μg/ml Tunicamycin treatment for 16 hr and 4 mM Benzyl glucosinolate treatment for 32 hr)

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glypican 1,glypican

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Secreted glypican-1: Secreted , extracellular space

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Glypican 1 antibody used in Western Blot (WB). GTX132672

GTX132672 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody (GTX132672) diluted at 1:500 and competitor's antibody (sc-365000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Glypican 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX132672

GTX132672 IHC-P Image

Glypican 1 antibody detects Glypican 1 protein at cell membrane in human pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreas, normal (left) and cancer (right).
Glypican 1 antibody (GTX132672) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glypican 1 antibody used in Western Blot (WB). GTX132672

GTX132672 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody (GTX132672) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glypican 1 antibody used in Western Blot (WB). GTX132672

GTX132672 WB Image

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody (GTX132672) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169