GeneTex

  • Country / Location Selection

United States (US)

Glypican-3 antibody [9C2]

Anti-Glypican-3 antibody [9C2] used in IHC (Paraffin sections) (IHC-P). GTX60603
Anti-Glypican-3 antibody [9C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60603
Anti-Glypican-3 antibody [9C2] used in Western Blot (WB). GTX60603
Anti-Glypican-3 antibody [9C2] used in Western Blot (WB). GTX60603
Anti-Glypican-3 antibody [9C2] used in Flow cytometry (FACS). GTX60603
Anti-Glypican-3 antibody [9C2] used in ELISA (ELISA). GTX60603
Anti-Glypican-3 antibody [9C2] used in IHC (Paraffin sections) (IHC-P). GTX60603

Cat. No. GTX60603

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9C2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Glypican-3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human GPC3 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glypican 3 , DGSX , GTR2-2 , MXR7 , OCI-5 , SDYS , SGB , SGBS , SGBS1

Cellular Localization

Cell membrane

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-Glypican-3 antibody [9C2] used in IHC (Paraffin sections) (IHC-P). GTX60603

GTX60603 IHC-P Image

IHC-P analysis of breast cancer tissue using GTX60603 Glypican-3 antibody [9C2].

Anti-Glypican-3 antibody [9C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60603

GTX60603 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60603 Glypican-3 antibody [9C2].
Green : Glypican-3
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-Glypican-3 antibody [9C2] used in Western Blot (WB). GTX60603

GTX60603 WB Image

WB analysis of HepG2 (1), HEK293 (2), Jurkat (3), SK-N-SH (4), PC-12 (5), F9 (6)and Mouse liver (7) cell lysate using GTX60603 Glypican-3 antibody [9C2].

Anti-Glypican-3 antibody [9C2] used in Western Blot (WB). GTX60603

GTX60603 WB Image

WB analysis of human Glypican-3 (AA: 55-200) recombinant protein using GTX60603 Glypican-3 antibody [9C2].

Anti-Glypican-3 antibody [9C2] used in Flow cytometry (FACS). GTX60603

GTX60603 FACS Image

FACS analysis of Jurkat cells using GTX60603 Glypican-3 antibody [9C2].
Green : Glypican-3
Red : negative control

Anti-Glypican-3 antibody [9C2] used in ELISA (ELISA). GTX60603

GTX60603 ELISA Image

ELISA analysis of antigen using GTX60603 Glypican-3 antibody [9C2].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-Glypican-3 antibody [9C2] used in IHC (Paraffin sections) (IHC-P). GTX60603

GTX60603 IHC-P Image

IHC-P analysis of liver cancer tissue using GTX60603 Glypican-3 antibody [9C2].

REFERENCE

There are currently no references for Glypican-3 antibody [9C2] (GTX60603). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Glypican-3 antibody [9C2] (GTX60603). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399