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Glypican-3 antibody [GT764]

Anti-Glypican-3 antibody [GT764] used in Western Blot (WB). GTX633303
Anti-Glypican-3 antibody [GT764] used in Flow cytometry (FACS). GTX633303

Cat No. GTX633303

Host

Mouse

Clonality

Monoclonal

Clone Name

GT764

Isotype

IgG2b

Application

WB, FACS

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

HepG2 , HepG2 (24 μg/ml Tunicamycin treatment for 16 hr)

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Glypican-3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888335

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glypican 3 , DGSX , GTR2-2 , MXR7 , OCI-5 , SDYS , SGB , SGBS , SGBS1

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Secreted glypican-3: Secreted , extracellular space

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Glypican-3 antibody [GT764] used in Western Blot (WB). GTX633303

GTX633303 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody [GT764] (GTX633303) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Glypican-3 antibody [GT764] used in Flow cytometry (FACS). GTX633303

GTX633303 FACS Image

Glypican-3 antibody [GT764] (GTX633303) detects Glypican-3 protein by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: Glypican-3 antibody [GT764] (GTX633303) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

REFERENCE

There are currently no references for Glypican-3 antibody [GT764] (GTX633303). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Glypican-3 antibody [GT764] (GTX633303). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169