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Glypican-3 antibody, Internal

Anti-Glypican-3 antibody, Internal used in Western Blot (WB). GTX47449
Anti-Glypican-3 antibody, Internal used in Western Blot (WB). GTX47449
Anti-Glypican-3 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX47449

Cat No. GTX47449

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

human placenta

PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human Glypican-3

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glypican 3 , DGSX , GTR2-2 , MXR7 , OCI-5 , SDYS , SGB , SGBS , SGBS1

Cellular Localization

Cell membrane

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-Glypican-3 antibody, Internal used in Western Blot (WB). GTX47449

GTX47449 WB Image

WB analysis of human placenta tissue using GTX47449 Glypican-3 antibody at 1μg/ml.

Anti-Glypican-3 antibody, Internal used in Western Blot (WB). GTX47449

GTX47449 WB Image

WB analysis of human placenta tissue using GTX47449 Glypican-3 antibody at 2.5μg/ml.

Anti-Glypican-3 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX47449

GTX47449 IHC-P Image

IHC-P analysis of human placenta tissue using GTX47449 Glypican-3 antibody at 4.0-8.0μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf