APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A recombinant fragment containing amino acids 511-580 of human glypican-3
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DGSX , GPC3 , GTR22 , MXR7 , OCI5 , SDYS , SGB , SGBS , SGBS1 , glypican 3 , Glypican 3
Cellular Localization
Cell membrane,Secreted,Extracellular space
Background
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
Database
Research Area
DATA IMAGES
![Anti-Glypican-3 antibody [SPM595] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34762](/templates/default/js/lightbox/images/loading.gif "Anti-Glypican-3 antibody [SPM595] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34762")
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GTX34762 ICC/IF Image
ICC/IF analysis of MeOH-fixed HepG2 cells using GTX34762 Glypican-3 antibody [SPM595].
Green : Primary antibody
Red : nucleus
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GTX34762 IHC-P Image
IHC-P analysis of human hepatocellular carcinoma tissue using GTX34762 Glypican-3 antibody [SPM595].
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GTX34762 IHC-P Image
IHC-P analysis of human hepatocellular carcinoma tissue using GTX34762 Glypican-3 antibody [SPM595].
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GTX34762 FACS Image
FACS analysis of PFA-fixed HepG2 cells using GTX34762 Glypican-3 antibody [SPM595].
Blue : Primary antibody
Red : Isotype control
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REFERENCE
There are currently no references for Glypican-3 antibody [SPM595] (GTX34762). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Glypican-3 antibody [SPM595] (GTX34762). Be the first to share your experience with this product.
![Anti-Glypican-3 antibody [SPM595] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34762](/upload/website/prouct_img/small/GTX34762/GTX34762_20200115_ICC-IF_1595_w_23051500_385.webp "Anti-Glypican-3 antibody [SPM595] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34762")
![Anti-Glypican-3 antibody [SPM595] used in IHC (Paraffin sections) (IHC-P). GTX34762](/upload/website/prouct_img/small/GTX34762/GTX34762_20200115_IHC-P_614_w_23051500_942.webp "Anti-Glypican-3 antibody [SPM595] used in IHC (Paraffin sections) (IHC-P). GTX34762")
![Anti-Glypican-3 antibody [SPM595] used in IHC (Paraffin sections) (IHC-P). GTX34762](/upload/website/prouct_img/small/GTX34762/GTX34762_20200115_IHC-P_615_w_23051500_949.webp "Anti-Glypican-3 antibody [SPM595] used in IHC (Paraffin sections) (IHC-P). GTX34762")
![Anti-Glypican-3 antibody [SPM595] used in Flow cytometry (FACS). GTX34762](/upload/website/prouct_img/small/GTX34762/GTX34762_20200115_FACS_1689_w_23051500_891.webp "Anti-Glypican-3 antibody [SPM595] used in Flow cytometry (FACS). GTX34762")
![Anti-Glypican-3 antibody [SPM595] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34762](/upload/website/prouct_img/normal/GTX34762/GTX34762_20200115_ICC-IF_1595_w_23051500_385.webp "Anti-Glypican-3 antibody [SPM595] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34762")
![Anti-Glypican-3 antibody [SPM595] used in IHC (Paraffin sections) (IHC-P). GTX34762](/upload/website/prouct_img/normal/GTX34762/GTX34762_20200115_IHC-P_614_w_23051500_942.webp "Anti-Glypican-3 antibody [SPM595] used in IHC (Paraffin sections) (IHC-P). GTX34762")
![Anti-Glypican-3 antibody [SPM595] used in IHC (Paraffin sections) (IHC-P). GTX34762](/upload/website/prouct_img/normal/GTX34762/GTX34762_20200115_IHC-P_615_w_23051500_949.webp "Anti-Glypican-3 antibody [SPM595] used in IHC (Paraffin sections) (IHC-P). GTX34762")
![Anti-Glypican-3 antibody [SPM595] used in Flow cytometry (FACS). GTX34762](/upload/website/prouct_img/normal/GTX34762/GTX34762_20200115_FACS_1689_w_23051500_891.webp "Anti-Glypican-3 antibody [SPM595] used in Flow cytometry (FACS). GTX34762")