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Glypican-3 antibody [rGPC3/863]

Anti-Glypican-3 antibody [rGPC3/863] used in Flow cytometry (FACS). GTX34764
Anti-Glypican-3 antibody [rGPC3/863] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34764
Anti-Glypican-3 antibody [rGPC3/863] used in Protein Array (Protein Array). GTX34764
Anti-Glypican-3 antibody [rGPC3/863] used in IHC (Paraffin sections) (IHC-P). GTX34764

Cat No. GTX34764

Host

Mouse

Clonality

Monoclonal

Clone Name

rGPC3/863

Isotype

IgG1

Application

ICC/IF, IHC-P, FACS, Protein Array

Reactivity

Human, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human GPC3 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DGSX , GPC3 , GTR22 , MXR7 , OCI5 , SDYS , SGB , SGBS , SGBS1 , glypican 3 , Glypican 3

Cellular Localization

Cell membrane,Secreted,Extracellular space

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-Glypican-3 antibody [rGPC3/863] used in Flow cytometry (FACS). GTX34764

GTX34764 FACS Image

FACS analysis of MeOH-fixed HepG2 cells using GTX34764 Glypican-3 antibody [rGPC3/863].
Blue : Primary antibody
Red : Isotype control

Anti-Glypican-3 antibody [rGPC3/863] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34764

GTX34764 ICC/IF Image

ICC/IF analysis of MeOH-fixed HepG2 cells using GTX34764 Glypican-3 antibody [rGPC3/863].
Green : Primary antibody
Red : nucleus

Anti-Glypican-3 antibody [rGPC3/863] used in Protein Array (Protein Array). GTX34764

GTX34764 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Mouse Glypican-3 Recombinant Monoclonal Antibody (rGPC3/863). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-Glypican-3 antibody [rGPC3/863] used in IHC (Paraffin sections) (IHC-P). GTX34764

GTX34764 IHC-P Image

IHC-P analysis of human hepatocellular carcinoma tissue using GTX34764 Glypican-3 antibody [rGPC3/863].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf