APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HepG2 (24 μg/ml Tunicamycin treatment for 16 hr)
Predict Reactivity
Mouse, Rat, Bovine, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.27 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Glypican-3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886331
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glypican 3 , DGSX , GTR2-2 , MXR7 , OCI-5 , SDYS , SGB , SGBS , SGBS1
Cellular Localization
Cell membrane; Lipid-anchor , GPI-anchor; Extracellular side , Secreted glypican-3: Secreted , extracellular space
Background
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
Database
Research Area
DATA IMAGES
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GTX130732 IHC-P Image
Glypican-3 antibody detects Glypican-3 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human pancreatic cancer.Glypican-3 stained by Glypican-3 antibody (GTX130732) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130732 WB Image
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody (GTX130732) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX130732 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GPC3 antibody (GTX130732) diluted at 1:1000.
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GTX130732 ICC/IF Image
Glypican-3 antibody detects Glypican-3 protein at cell membrane by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glypican-3 protein stained by Glypican-3 antibody (GTX130732) diluted at 1:400.
Blue: Hoechst 33342 staining.
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REFERENCE
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