GeneTex
United States (US)

Glypican 4 antibody [AT51E3]

GTX16498_WB_18121410_931.jpg
GTX16498_ICCIF_18121410_145.jpg
GTX16498_FACS_18121410_895.jpg

Cat No. GTX16498

Host Mouse
Clonality Monoclonal
Clone Name AT51E3
Isotype IgG2a
Application WB, ICC/IF, FACS, ELISA
Reactivity Human
APPLICATION

Application Note

WB: 1:1000. ICC/IF: 1:200. FACS: 1:200

Calculated MW

62 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human GPC4 (401-529aa)

Purification

By protein-A affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glypican 4,K-Glypican,Gpc4

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX16498_WB_18121410_931.jpg

GTX16498 WB Image

WB analysis of (1) recombinant human GPC4 (50ng) and (2) Ramos lysate (40ug) using GPC4 antibody [AT51E3] at a dilution of 1:1000.

GTX16498_ICCIF_18121410_145.jpg

GTX16498 ICC/IF Image

ICC/IF analysis of 293T cells using DAPI (blue) and GPC4 antibody [AT51E3] at a dilution of 1:200 (green).

GTX16498_FACS_18121410_895.jpg

GTX16498 FACS Image

FACS analysis of 293T cells using an isotype control (black) or GPC4 antibody [AT51E3] at 2-5ug for 1,000,000 cells (red).

Package List Price ($)
$ 319