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GnRHR antibody [HL2638]

Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094
Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094
Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094
Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094
Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094
Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094
Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094
Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094

Cat. No. GTX639094

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2638

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Product Note

This antibody was raised against human GnRHR Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Extracellular domain of human GnRHR. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gonadotropin releasing hormone receptor , GNRHR1 , GRHR , HH7 , LHRHR , LRHR

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes the receptor for type 1 gonadotropin-releasing hormone. This receptor is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Following binding of gonadotropin-releasing hormone, the receptor associates with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Activation of the receptor ultimately causes the release of gonadotropic luteinizing hormone (LH) and follicle stimulating hormone (FSH). Defects in this gene are a cause of hypogonadotropic hypogonadism (HH). Alternative splicing results in multiple transcript variants encoding different isoforms. More than 18 transcription initiation sites in the 5' region and multiple polyA signals in the 3' region have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094

GTX639094 WB Image

Non-transfected (–) and transfected (+) unboiled PC-3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GnRHR antibody [HL2638] (GTX639094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094

GTX639094 WB Image

Unboiled PC-3 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with GnRHR antibody [HL2638] (GTX639094) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094

GTX639094 IHC-P Image

GnRHR antibody [HL2638] detects GnRHR protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded PC-3 xenograft.
GnRHR stained by GnRHR antibody [HL2638] (GTX639094) diluted at 1:200 and competitor's antibody (19950-1-AP) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094

GTX639094 WB Image

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GnRHR antibody [HL2638] (GTX639094) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GnRHR antibody [HL2638] used in Western Blot (WB). GTX639094

GTX639094 WB Image

Boiled and unboiled PC-3 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GnRHR antibody [HL2638] (GTX639094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094

GTX639094 IHC-P Image

GnRHR antibody [HL2638] detects GnRHR protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human PC-3 xenograft.
GnRHR stained by GnRHR antibody [HL2638] (GTX639094) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094

GTX639094 IHC-P Image

GnRHR antibody [HL2638] detects GnRHR protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
GnRHR stained by GnRHR antibody [HL2638] (GTX639094) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GnRHR antibody [HL2638] used in IHC (Paraffin sections) (IHC-P). GTX639094

GTX639094 IHC-P Image

GnRHR antibody [HL2638] detects GnRHR protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
GnRHR stained by GnRHR antibody [HL2638] (GTX639094) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations