GnRHR antibody [HL2638]

Non-transfected (–) and transfected (+) unboiled PC-3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GnRHR antibody [HL2638] (GTX639094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled PC-3 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GnRHR antibody [HL2638] (GTX639094) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GnRHR antibody [HL2638] (GTX639094) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.