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Goat Anti-Mouse IgG antibody, pre-adsorbed (FITC)

Goat Anti-Mouse IgG antibody, pre-adsorbed (FITC). GTX27064
Goat Anti-Mouse IgG antibody, pre-adsorbed (FITC). GTX27064
Goat Anti-Mouse IgG antibody, pre-adsorbed (FITC). GTX27064

Cat. No. GTX27064

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, Dot, ELISA, IHC

Reactivity

 Mouse
Package
100 μg ($129)
See all Mouse IgG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:1000-1:5000
FACS 1:500-1:2500
Dot Assay dependent
ELISA 1:10000-1:50000
IHC Assay dependent
Not tested in other applications.

Product Note

Pre-adsorbed with Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep serum proteins. May react with immunoglobulins from other species.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse IgG whole molecule

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities.
From serum

Conjugation

Fluorescein isothiocyanate (FITC)
Ratio : 3.08 molecules FITC per Goat IgG molecule.

RRID

AB_378074

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Goat Anti-Mouse IgG antibody, pre-adsorbed (FITC). GTX27064

GTX27064 Dot Image

Dot Blot showing the detection of Mouse IgG (GTX27064). A three-fold serial dilution of Mouse IgG starting at 200ng was spotted onto 0.45 um nitrocellulose. After blocking, GTX27064 was used at 1:1000.

Goat Anti-Mouse IgG antibody, pre-adsorbed (FITC). GTX27064

GTX27064 WB Image

WB analysis of mouse IgG using GTX27064 Goat Anti-Mouse IgG antibody, pre-adsorbed (FITC).
Loading : 50 ng
Dilution : 1:1000

Goat Anti-Mouse IgG antibody, pre-adsorbed (FITC). GTX27064

GTX27064 Dot Image

Dot blot analysis of mouse IgG using GTX27064 Goat Anti-Mouse IgG antibody, pre-adsorbed (FITC).
Dilution : 1:1000

REFERENCE

Application Reference

Qin Z et al. J Exp Clin Cancer Res 2019; 38 (1) : 154 Transketolase (TKT) activity and nuclear localization promote hepatocellular carcinoma in a metabolic and a non-metabolic manner.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 129