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Goat Anti-Mouse IgG antibody, pre-adsorbed (Rhodamine)

Cat. No. GTX27065

Reference 2

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, FACS, Dot, ELISA, FISH, Multiplexing

Reactivity

 Mouse
Package
100 μg ($149)
See all Mouse IgG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:1000-1:5000
FACS 1:500-1:2500
Dot Assay dependent
ELISA 1:10000-1:50000
FISH Assay dependent
Multiplexing Assay dependent
Not tested in other applications.

Product Note

Pre-adsorbed with Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep serum proteins. May react with immunoglobulins from other species.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse IgG whole molecule

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities.
From serum

Conjugation

Rhodamine
Ratio : 1.0 molecules Rhodamine per Goat IgG molecule.

RRID

AB_378075

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

REFERENCE

Application Reference

Matsubara S et al. J Immunother Cancer 2019; 7 (1) : 256 Tertiary lymphoid organs in the inflammatory myopathy associated with PD-1 inhibitors.
Matsubara S et al. Neuromuscular Disorders 2018; (Epub) Mitophagy in three cases of immune-mediated necrotizing myopathy associated with anti-3-hydroxy-3-methylglutaryl-coenzyme a reductase autoantibodies. ultrastructural and immunohistochemical studies.
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 149