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Goat Anti-Mouse IgG2c antibody (HRP)

Goat Anti-Mouse IgG2c antibody (HRP). GTX77297

Cat No. GTX77297

Reference 4

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, IHC

Reactivity

 Mouse
Package
500 μg ($179)
See all Mouse IgG2c products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Specificity/Sensitivity

This product reacts with IgG 2c in C57BL/6, SJL, C57BL/10, CB20, C57BL/6 by Balb/C crosses and pools of serum of outbred mice. The anti IgG 2c does not react with L-chains or IgG 2a, IgG 2b, IgG 1 or IgG3.This reagent may also be used where a reagent specific for Igh 1-b allele is indicated. (Reference: J. Immunological Methods 212: 187-192, 1998).

PROPERTIES

Form

Liquid

Buffer

50 mM HEPES pH 7.0, 0.1 M sodium chloride, 0.2% BSA, 0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4 ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Goats were immunized with highly purified IgG 2c isolated from C57BL/6 mice, in Freund's adjuvant.

Target Isotype

IgG2c

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

DATA IMAGES

Goat Anti-Mouse IgG2c antibody (HRP). GTX77297

GTX77297 IHC Image

REFERENCE

Application Reference

Jesus S et al. Mol Pharm 2017; (Epub) Adjuvant Activity of Poly-ε-caprolactone/Chitosan Nanoparticles Characterized by Mast Cell Activation and IFN-γ and IL-17 Production.
Wegmann F et al. Clin Vaccine Immunol 2015; 22 (9) : 1004-12 The Carbomer-Lecithin Adjuvant Adjuplex Has Potent Immunoactivating Properties and Elicits Protective Adaptive Immunity against Influenza Virus Challenge in Mice.
Sheppard NC et al. Int Immunol 2014; Polyethyleneimine is a potent systemic adjuvant for glycoprotein antigens.
Application : ELISA
Reactivity : Mouse
Moghaddam AE et al. J Immunol 2011; 187 (4) : 1626-33 Reactive carbonyls are a major Th2-inducing damage-associated molecular pattern generated by oxidative stress.
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SDS
Thimerosal.pdf
Package List Price ($)
$ 179