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Goat Anti-Rabbit IgG antibody (DyLight488)

Goat Anti-Rabbit IgG antibody (DyLight488). GTX213110-04
Goat Anti-Rabbit IgG antibody (DyLight488). GTX213110-04
Goat Anti-Rabbit IgG antibody (DyLight488). GTX213110-04
Goat Anti-Rabbit IgG antibody (DyLight488). GTX213110-04
Goat Anti-Rabbit IgG antibody (DyLight488). GTX213110-04
Goat Anti-Rabbit IgG antibody (DyLight488). GTX213110-04

Cat No. GTX213110-04

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, FACS

Reactivity

Rabbit
Package
500 μl ($149)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC-Wm 1:100-1:500
FACS 1:50-1:1000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Highly purified whole rabbit IgG

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography.

Conjugation

DyLight488

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Goat Anti Rabbit IgG

DATA IMAGES

Goat Anti-Rabbit IgG antibody (DyLight488). GTX213110-04

GTX213110-04 IHC-P Image

Double-labeled immunofluorescence photomicrographs of paraffin-embedded sections of mouse colon.
Green: E-Cadherin antibody (GTX100443) diluted at 1:500. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05).
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Goat Anti-Rabbit IgG antibody (DyLight488). GTX213110-04

GTX213110-04 IHC-Wm Image

Whole mount immunohistochemical analysis of paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo using Pax2a antibody (GTX128127) detected by anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
GTX128127 diluted at 1:100 and incubated overnight at 4ºC.
GTX213110-04 diluted at 1:500 and incubated 3 hours at room temperature.

Goat Anti-Rabbit IgG antibody (DyLight488). GTX213110-04

GTX213110-04 IHC-Fr Image

Double-labeled immunofluorescence photomicrographs of frozen sections of mouse brain.
Green: Vimentin antibody (GTX100619) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Red: ME1 antibody [GT15611] (GTX632190) diluted at 1:200. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05).
Blue: Nuclear staining with Hoechst 33342.

Goat Anti-Rabbit IgG antibody (DyLight488). GTX213110-04

GTX213110-04 FACS Image

CD81 antibody (GTX101766) detects CD81 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: CD81 antibody (GTX101766) dilution: 1:50.
The Rabbit IgG antibody (DyLight488) (GTX213110-04) was used to detect the primary antibody.

Goat Anti-Rabbit IgG antibody (DyLight488). GTX213110-04

GTX213110-04 FACS Image

Glypican 1 antibody [N3C3] (GTX104557) detects Glypican 1 protein by flow cytometry analysis.
Sample: A431 cell.
Black: Unlabelled sample was used as a control.
Red: Glypican 1 antibody [N3C3] (GTX104557) dilution: 1:50.
Acquisition of 20,000 events were collected using the Rabbit IgG (DyLight488) (GTX113110-04) secondary antibody for FACS analysis.

Goat Anti-Rabbit IgG antibody (DyLight488). GTX213110-04

GTX213110-04 ICC/IF Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
The signal was developed using Goat Anti-Rabbit IgG antibody (DyLight488) (GTX213110-04) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 149