Goat Anti-Rabbit IgG antibody (DyLight594)

Cat. No. GTX213110-05

Reference 12

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS
Reactivity	Rabbit
Package	500 μl ($149)

See all Rabbit IgG products

View product citations for antibody GTX213110-05 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	1:100-1:2000
IHC-P	Assay dependent
IHC-Fr	1:100-1:1000
FACS	1:50-1:200

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Highly purified whole rabbit IgG

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography.

Conjugation

DyLight594

RRID

AB_2887580

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Goat Anti Rabbit IgG

DATA IMAGES

GTX213110-05 ICC/IF Image

beta Tubulin antibody detects beta Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: alpha Tubulin stained by alpha Tubulin antibody (GTX101279) diluted at 1:100.
The signal was developed using Goat Anti-Rabbit IgG antibody (DyLight594) (GTX213110-05) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX213110-05 FACS Image

BrdU antibody (GTX128091) detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4ºC for 15 minutes by flow cytometry analysis.
Black: Unlabelled sample was used as a control.
Red: BrdU antibody (GTX128091) dilution: 1:50.
The Rabbit IgG antibody (DyLight594) (GTX213110-05) was used to detect the primary antibody.

GTX213110-05 IHC-Fr Image

Immunofluorescence photomicrographs of frozen sections of mouse liver.
Red: Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight594) (GTX213110-05).
Blue: Nuclear staining with Hoechst 33342.

GTX213110-05 ICC/IF Image

beta Tubulin antibody detects beta Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Red: alpha Tubulin stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
The signal was developed using Rabbit IgG antibody (DyLight594) (GTX213111-05) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Xue B et al. Sci Rep 2024; 14 (1) : 837 Circulating exosomal miR-16-5p and let-7e-5p are associated with bladder fibrosis of diabetic cystopathy.

Bu KB et al. Int J Mol Sci 2024; 25 (2) Regulation of Benzo[a]pyrene-Induced Hepatic Lipid Accumulation through CYP1B1-Induced mTOR-Mediated Lipophagy.

Y Chen et al. Neurobiol Dis 2023; 188 : 106346 Neuronal MeCP2 in the dentate gyrus regulates mossy fiber sprouting of mice with temporal lobe epilepsy

LM Tseng et al. Exp Cell Res 2023; : 113652 Regorafenib induces damage-associated molecular patterns, cancer cell death and immune modulatory effects in a murine triple negative breast cancer model

Fasano G et al. Nat Commun 2022; 13 (1) : 6841 Dominant ARF3 variants disrupt Golgi integrity and cause a neurodevelopmental disorder recapitulated in zebrafish.

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REVIEW

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Application Reference

Xue B et al. Sci Rep 2024; 14 (1):837 Circulating exosomal miR-16-5p and let-7e-5p are associated with bladder fibrosis of diabetic cystopathy.

Bu KB et al. Int J Mol Sci 2024; 25 (2) Regulation of Benzo[a]pyrene-Induced Hepatic Lipid Accumulation through CYP1B1-Induced mTOR-Mediated Lipophagy.

Y Chen et al. Neurobiol Dis 2023; 188:106346 Neuronal MeCP2 in the dentate gyrus regulates mossy fiber sprouting of mice with temporal lobe epilepsy

LM Tseng et al. Exp Cell Res 2023;:113652 Regorafenib induces damage-associated molecular patterns, cancer cell death and immune modulatory effects in a murine triple negative breast cancer model

Fasano G et al. Nat Commun 2022; 13 (1):6841 Dominant ARF3 variants disrupt Golgi integrity and cause a neurodevelopmental disorder recapitulated in zebrafish.

Wang IH et al. Cancers (Basel) 2020; 12 (5) Mevalonate Pathway Enzyme HMGCS1 Contributes to Gastric Cancer Progression.

Ou J et al. Cell Mol Biol Lett 2019; 24:39 Non-contact co-culture with human vascular endothelial cells promotes epithelial-to-mesenchymal transition of cervical cancer SiHa cells by activating the NOTCH1/LOX/SNAIL pathway.

Yao-Shen Huang et al. bioRxiv 2019; (Epub) Sonogenetic modulation of cellular activities using an engineered auditory-sensing protein.

Wu JY et al. Cancers (Basel) 2019; 11 (5) YC-1 Antagonizes Wnt/帣-Catenin Signaling Through the EBP1 p42 Isoform in Hepatocellular Carcinoma.

Jing Q et al. J Cell Mol Med 2019; 23 (7):4711-4722 Wnt3a promotes radioresistance via autophagy in squamous cell carcinoma of the head and neck.