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Goat Anti-Rabbit IgG antibody, pre-adsorbed (HRP)

Goat Anti-Rabbit IgG antibody, pre-adsorbed (HRP). GTX27090
Goat Anti-Rabbit IgG antibody, pre-adsorbed (HRP). GTX27090
Goat Anti-Rabbit IgG antibody, pre-adsorbed (HRP). GTX27090
Goat Anti-Rabbit IgG antibody, pre-adsorbed (HRP). GTX27090
Goat Anti-Rabbit IgG antibody, pre-adsorbed (HRP). GTX27090

Cat. No. GTX27090

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, Dot, ELISA, IHC

Reactivity

 Rabbit
Package
100 μg ($129)
See all Rabbit IgG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000-1:10000
Dot Assay dependent
ELISA 1:20000-1:100000
IHC 1:1000-1:5000
Not tested in other applications.

Product Note

Pre-adsorbed with Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep serum proteins. May react with immunoglobulins from other species.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Gentamicin Sulfate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rabbit

Immunogen

Rabbit IgG whole molecule

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities.
From serum

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Goat Anti-Rabbit IgG antibody, pre-adsorbed (HRP). GTX27090

GTX27090 WB Image

GTX27090 secondary antibody was used at 1:40,000 in blocking buffer to detect a rabbit primary antibody by Western Blot. Anti p27 antibody (GTX48799, 1:1000 RT 30 minutes) showed detection of 0.1 μg of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose and blocked with 1% BSA-TTBS overnight at 4ºC. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).

Goat Anti-Rabbit IgG antibody, pre-adsorbed (HRP). GTX27090

GTX27090 Dot Image

GeneTex secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein 250ng starting total load Co-incubated with GeneTex HRP conjugated Goat anti Rabbit IgG antibody (GTX27090) and Dylight 649 conjugated goat anti Rabbit IgG antiody at 1:20K in blocking buffer.

Goat Anti-Rabbit IgG antibody, pre-adsorbed (HRP). GTX27090

GTX27090 ELISA Image

ELISA analysis of various samples using serially diluted GTX27090 Goat Anti-Rabbit IgG antibody, pre-adsorbed (HRP).
Red : Rabbit IgG
Green : Bovine IgG
Blue : Chicken IgG
Purple : Goat IgG
Coating : 10 μg

Goat Anti-Rabbit IgG antibody, pre-adsorbed (HRP). GTX27090

GTX27090 WB Image

WB analysis of various samples using anti-p27 antibody followed by GTX27090 Goat Anti-Rabbit IgG antibody, pre-adsorbed (HRP).
Lane 1 : Protein ladder
Lane 2 : MBP-p27 protein
Lane 3 : MBP
Dilution : 1:40000

Goat Anti-Rabbit IgG antibody, pre-adsorbed (HRP). GTX27090

GTX27090 Dot Image

Dot blot analysis of serially diluted rabbit IgG using GTX27090 Goat Anti-Rabbit IgG antibody, pre-adsorbed (HRP) detected by different platforms.
Dilution : 1:20000

REFERENCE

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REVIEW

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Package List Price ($)
$ 129