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Goat Anti-Rat IgG (F(ab')2) antibody, F(ab')2 fragment, pre-adsorbed (FITC)

Cat. No. GTX26266

Host

Goat

Clonality

Polyclonal

Application

WB, ICC/IF, FACS, ELISA

Reactivity

Rat
Package
500 μg ($179)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:50 - 1:200
ICC/IF 1:50 - 1:200
FACS 1:500 - 1:2,500
ELISA 1:50 - 1:200
Not tested in other applications.

Product Note

This antibody has been pre-adsorbed against Bovine, Horse and Human serum proteins to ensure minimal cross-reactivity. Cross-reactivity with other species, however, is possible.

PROPERTIES

Form

Liquid

Buffer

0.01M Sodium Phosphate pH7.2, 0.25M Sodium Chloride, 15mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Rat IgG F(ab')2 fragment

Target Isotype

IgG F(ab')2

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 179