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Goat Anti-Rat IgG antibody (PE)

Cat No. GTX72984

Host

Goat

Clonality

Polyclonal

Application

FACS

Reactivity

Rat
Package
1 ml ($259)

APPLICATION

Application Note

Prepare conjugate as described above. Use 1 to 3 micrograms of the conjugate per 1 x 106 cells suspended in 0.1 mL. This suspension contains 1 mg of conjugate per milliliter. Do not dilute entire sample at once. Mix vial contents well. Then, withdraw aliquots as needed with a micropipette and transfer into a conical centrifuge tube. Centrifuge at maximum speed for 4 minutes. Remove and discard clear supernatant and disslove colored precipitate in 100 mM sodium phosphate pH 7.0 containing 5 mg/mL of bovine serum albumin. This suspension contains 1 mg of conjugate per milliliter. Do not dilute entire sample at once. Mix vial contents well. Then, withdraw aliquots as needed with a micropipette and transfer into a conical centrifuge tube. Centrifuge at maximum speed for 4 minutes. Remove and discard clear supernatant and disslove colored precipitate in 100 mM sodium phosphate pH 7.0 containing 5 mg/mL of bovine serum albumin. PROTEIN CARRIER: bovine serum albumin CONJUGATE CHARACTERISTICS: The antibody used in this product is an affinity-purified goat anti rat IgG with heavy and light chain specificity. This antibody does not cross react with human immunoglobulins. This antibody cross reacts strongly with mouse IgG. Total activity of conjugate against immobilized rat gammaglobulins: >95% The biliprotein used in making this conjugate had the following characteristics: Mol.Wt. 240,000 Molar Extinction Coefficient at 565 nm: 1.9 x 106 Emission Max. 575 nm (using excitation at 500 nm). LICENSED FOR USE IN FLOW CYTOMETRY.

PROPERTIES

Form

Liquid

Buffer

3.0 M Ammonium Sulfate, 50 mM Sodium Phosphate, 0.05% Sodium Azide, pH 7.0.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Target Isotype

IgG

Conjugation

Phycoerythrin (PE)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 259