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Goat IgG isotype control

Goat IgG isotype control. GTX35039

Cat No. GTX35039

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

IHC-P, FACS, IP, ELISA, IHC
Package
100 μg ($79)

APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

PROPERTIES

Form

Liquid

Buffer

100 mM borate buffered saline, pH 8.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Isotype control for Goat IgG, Isotype control to Goat IgG, Polyclonal Goat IgG isotype control, Goat negative control for IgG, Goat IgG negative control, Goat IgG isotype control, Goat isotype control for IgG, Negative control for Goat IgG

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

DATA IMAGES

Goat IgG isotype control. GTX35039

GTX35039 IP Image

Immunoprecipitation of Rock-2 protein from HeLa whole cell extracts using 5 μg goat IgG isotype control (GTX35039) or Rock-2 Goat pAb.
Western blot analysis was performed using Rock-2 goat pAb.
Goat IgG HRP (GTX228416-01) was used as a secondary reagent.

REFERENCE

REVIEW

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Package List Price ($)
$ 79