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Goat IgG isotype control

Goat IgG isotype control. GTX35039

Cat. No. GTX35039

Reference 9

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 FACS, IP, ELISA, IHC
Package
100 μg ($119)
See all Goat IgG isotype control products

APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

PROPERTIES

Form

Liquid

Buffer

Borate buffered saline

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Isotype control for Goat IgG , Isotype control to Goat IgG , Polyclonal Goat IgG isotype control , Goat negative control for IgG , Goat IgG negative control , Goat IgG isotype control , Goat isotype control for IgG , Negative control for Goat IgG

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

DATA IMAGES

Goat IgG isotype control. GTX35039

GTX35039 IP Image

Immunoprecipitation of Rock-2 protein from HeLa whole cell extracts using 5 μg goat IgG isotype control (GTX35039) or Rock-2 Goat pAb.
Western blot analysis was performed using Rock-2 goat pAb.
Goat IgG HRP (GTX228416-01) was used as a secondary reagent.

REFERENCE

Application Reference

Lai JH et al. Sci Rep 2023; 13 (1) : 20126 USP18 enhances dengue virus replication by regulating mitochondrial DNA release.
Jaco Botha et al. J Extracell Vesicles 2022; 11 (4) : e12200 Lipid-based strategies used to identify extracellular vesicles in flow cytometry can be confounded by lipoproteins: Evaluations of annexin V, lactadherin, and detergent lysis.
Wojciech J Szlachcic et al. iScience 2022; 25 (7) : 104594 SARS-CoV-2 infects an in?vitro model of the human developing pancreas through endocytosis.
Jenn-Haung Lai et al. iScience 2021; 24 (6) : 102498 Mitochondrial CMPK2 mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways.
Naskalska A et al. J Virol 2019; Membrane protein of HCoV-NL63 is responsible for interaction with the adhesion receptor.
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Package List Price ($)
$ 119