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Goat Lactoferrin protein (active)

Goat Lactoferrin protein (active). GTX00078-pro
Goat Lactoferrin protein (active). GTX00078-pro
Goat Lactoferrin protein (active). GTX00078-pro

Cat No. GTX00078-pro

Application Functional Assay
Species Goat
Package
10 μg ($289)
APPLICATION

Application Note

Lactotransferrin (LTF), also known as actoferrin (LF) , is a multifunctional protein of the transferrin family. Lactoferrin belongs to the innate immune system. Apart from its main biological function, namely binding and transport of iron ions, lactoferrin also has antibacterial, antiviral, antiparasitic, catalytic, anti-cancer, and anti-allergic functions and properties. LTF is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions. It also present in secondary granules of PMN and is secreted by some acinar cells. Besides, Clusterin (CLU) has been identified as an interactor of LTF, thus a binding ELISA assay was conducted to detect the interaction of recombinant goat LTF and recombinant goat CLU. Briefly, LTF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CLU-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-LTF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of LTF and CLU was in a dose dependent manner.

Observed MW

37 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Val25~Glu352 (NP_001272477.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

If

Database

Research Area

DATA IMAGES
Goat Lactoferrin protein (active). GTX00078-pro

GTX00078-pro Functional Assay Image

Functional ELISA analysis of GTX00078-pro Goat Lactoferrin protein (active) which can bind immobilized CLU protein.

Goat Lactoferrin protein (active). GTX00078-pro

GTX00078-pro Image

SDS-PAGE analysis of GTX00078-pro Goat Lactoferrin protein (active).

Goat Lactoferrin protein (active). GTX00078-pro

GTX00078-pro Image

WB analysis of GTX00078-pro Goat Lactoferrin protein (active).

REFERENCE
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