GeneTex
United States (US)

Granulins antibody, Internal

GTX88443_2239_WB_18121411_704.jpg

Cat No. GTX88443

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88443-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence QSKCLSKENATTD , from the internal region of the protein sequence according to NP_002078.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CLN11 Antibody , PCDGF Antibody , PGRN Antibody , GRN Antibody , GP88 Antibody , GEP Antibody , PEPI Antibody

Background

Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. [provided by RefSeq, Jul 2008]
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Database

Research Area

DATA IMAGES
GTX88443_2239_WB_18121411_704.jpg

GTX88443 WB Image

WB analysis of HeLa lysate using GTX88443 Granulins antibody, Internal.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer