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Granulins antibody

Comparison Orthogonal
Anti-Granulins antibody used in Western Blot (WB). GTX100803
Anti-Granulins antibody used in IHC (Paraffin sections) (IHC-P). GTX100803
Anti-Granulins antibody used in Western Blot (WB). GTX100803

Cat. No. GTX100803

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all Granulins products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

HepG2 conditional medium

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Granulins. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240920

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

granulin precursor , CLN11 , GEP , GP88 , PCDGF , PEPI , PGRN

Cellular Localization

Secreted

Background

Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Granulins antibody used in Western Blot (WB). GTX100803

GTX100803 WB Image

Comparison Orthogonal

HepG2 conditioned medium (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with Granulins antibody (GTX100803) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Granulins antibody used in IHC (Paraffin sections) (IHC-P). GTX100803

GTX100803 IHC-P Image

Granulins antibody detects GRN protein at cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Granulins antibody (GTX100803) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Granulins antibody used in Western Blot (WB). GTX100803

GTX100803 WB Image

Orthogonal

HepG2 conditioned medium (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Granulins antibody (GTX100803) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Kahle JJ et al. Hum Mol Genet 2011; 20 (3) : 510-27 Comparison of an expanded ataxia interactome with patient medical records reveals a relationship between macular degeneration and ataxia.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169