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Granzyme A antibody [N2C3]

Anti-Granzyme A antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114461
Anti-Granzyme A antibody [N2C3] used in Western Blot (WB). GTX114461

Cat. No. GTX114461

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all Granzyme A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

A549 , HeLa , HCT116

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Granzyme A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619575

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

granzyme A , CTLA3 , HFSP

Cellular Localization

Secreted , Cytoplasmic granule

Background

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Granzyme A antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX114461

GTX114461 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Granzyme A(GTX114461) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Granzyme A antibody [N2C3] used in Western Blot (WB). GTX114461

GTX114461 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: Hela
C: HCT116
12% SDS PAGE
Granzyme A antibody
GTX114461 diluted at 1:1000

REFERENCE

Application Reference

Jong AY et al. J Extracell Vesicles 2017; 6 (1) : 1294368 Large-scale isolation and cytotoxicity of extracellular vesicles derived from activated human natural killer cells.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169