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Granzyme B antibody [GB11]

Anti-Granzyme B antibody [GB11] used in ELISA (ELISA). GTX75843

Cat. No. GTX75843

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GB11

Isotype

 IgG1

Application

 ICC/IF, IHC-Fr, FACS, IP, ELISA

Reactivity

 Human, Monkey, Chimpanzee
Package
50 μg ($399)
See all Granzyme B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS 1/10-1/100
IP Assay dependent
ELISA 2-5μg/ml

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Product Note

This antibody has been reported to function as a capture reagent in sandwich ELISA assays for soluble Granzyme B in conjunction with biotin conjugated GB10 (GTX75842) as detection reagent.

PROPERTIES

Form

Liquid

Buffer

TBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human Granzyme B.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_377065

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

granzyme B , C11 , CCPI , CGL-1 , CGL1 , CSP-B , CSPB , CTLA1 , CTSGL1 , HLP , SECT

Cellular Localization

Cytoplasmic granule

Background

This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]

Database

Research Area

DATA IMAGES

Anti-Granzyme B antibody [GB11] used in ELISA (ELISA). GTX75843

GTX75843 ELISA Image

Sandwich ELISA analysis of human granzyme B using Mouse anti Human granzyme B (GTX75843) as a capture reagent and biotinylated Mouse anti Human granzyme B (GTX75842) as a detection reagent. Human plasma samples are diluted i:2 (green) and 1:4 (blue).

REFERENCE

Application Reference

Nishie M et al. Cancer Immunol Immunother 2020; Downregulated ATP6V1B1 expression acidifies the intracellular environment of cancer cells leading to resistance to antibody-dependent cellular cytotoxicity.
Application : ICC/IF
Reactivity : Human
McNeel DG et al. Clin Cancer Res 2014; Real-Time Immune Monitoring to Guide Plasmid DNA Vaccination Schedule Targeting Prostatic Acid Phosphatase (PAP) in Patients with Castration-Resistant Prostate Cancer.
Application : ELISA
Reactivity : Human
Clayberger C et al. J Immunol 2012; 188 (12) : 6119-26 15 kDa granulysin causes differentiation of monocytes to dendritic cells but lacks cytotoxic activity.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399