GeneTex
  • Country / Location Selection

United States (US)

Granzyme B antibody [GB11]

Anti-Granzyme B antibody [GB11] used in ELISA (ELISA). GTX75843

Cat No. GTX75843

Host

Mouse

Clonality

Monoclonal

Clone Name

GB11

Isotype

IgG1

Application

ICC/IF, IHC-Fr, FACS, IP, ELISA

Reactivity

Human, Monkey, Chimpanzee
Package
50 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS 1/10-1/100
IP Assay dependent
ELISA 2-5μg/ml

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.

Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Specificity/Sensitivity

This antibody has been reported to function as a capture reagent in sandwich ELISA assays for soluble Granzyme B in conjunction with biotin conjugated GB10 (GTX75842) as detection reagent.

PROPERTIES

Form

Liquid

Buffer

Tris buffered saline, 0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human Granzyme B.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Granzyme B , C11 , Ccpi , Cgl-1 , Cgl1 , Csp-B , Cspb , Ctla1 , Ctsgl1 , Hlp , Sect , Gzmb

Background

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Granzyme B antibody [GB11] used in ELISA (ELISA). GTX75843

GTX75843 ELISA Image

Sandwich ELISA analysis of human granzyme B using Mouse anti Human granzyme B (GTX75843) as a capture reagent and biotinylated Mouse anti Human granzyme B (GTX75842) as a detection reagent. Human plasma samples are diluted i:2 (green) and 1:4 (blue).

REFERENCE

REVIEW

There are currently no reviews for Granzyme B antibody [GB11] (GTX75843). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 319