GeneTex
United States (US)

Growth Hormone antibody [N1C3]

GTX101220_IHC_19011506_479.jpg
GTX101220_WB_19011506_287.jpg
GTX101220_39967_IHC2_19011506_337.jpg

Cat No. GTX101220

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A431 , HeLaS3 , Raji

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Growth Hormone. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Growth Hormone 1,Gh,Gh-N,Ghb5,Ghn,Ighd1B,Hgh-N,Gh1

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101220_IHC_19011506_479.jpg

GTX101220 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Growth Hormone(GTX101220) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101220_WB_19011506_287.jpg

GTX101220 WB Image

Sample(30 µg whole cell lysate)
A:293T
12% SDS PAGE
GTX101220 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101220_39967_IHC2_19011506_337.jpg

GTX101220 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human placenta, using Growth Hormone(GTX101220) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169