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Grp75 antibody [30A5]

Anti-Grp75 antibody [30A5] used in Immunohistochemistry (IHC). GTX13529
Anti-Grp75 antibody [30A5] used in Western Blot (WB). GTX13529

Cat. No. GTX13529

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 30A5

Isotype

 IgG1

Application

 WB, ICC/IF, IP, IHC

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Hamster, Guinea pig, Pig, Monkey, Xenopus laevis
Package
25 μl ($399)
See all Grp75 products

APPLICATION

Application Note

Immunohistochemistry (1:50) Western Blot (1:1000, colorimetric) Optimal conditions must be determined individually for each application.

Calculated MW

74 kDa. ( Note )

Positive Control

HeLa Cell Lysate (Heat Shocked)

Product Note

This antibody detects an ~75 kDa protein, corresponding to the apparent molecular mass of Glucose Regulated Protein 75 (Grp75) on SDS-PAGE immunoblots.

PROPERTIES

Form

Liquid

Buffer

50% Glycerol, PBS pH7.2, 0.1mM PMSF

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Grp75 isolated from human Molt4 cells.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 9 , CRP40 , CSA , EVPLS , GRP-75 , GRP75 , HEL-S-124m , HSPA9B , MOT , MOT2 , MTHSP75 , PBP74 , SAAN , SIDBA4

Cellular Localization

Mitochondrion , Nucleolus

Background

This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.[provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-Grp75 antibody [30A5] used in Immunohistochemistry (IHC). GTX13529

GTX13529 IHC Image

Immunohistochemistry analysis of human breast cancer tissue immunohistochemically stained using Grp75 mAb (30A5).

Anti-Grp75 antibody [30A5] used in Western Blot (WB). GTX13529

GTX13529 WB Image

Western blot analysis of Grp75/Mortalin, mAb (30A5) (GTX13529):
Lane 1: MW marker
Lane 2: HeLa (heat shocked)
Lane 3: L-929 (heat shocked)
Lane 4: CHO -K1 (heat shocked)
Lane 5: GPC-16 (heat shocked)
Lane 6: PC-12 (heat shocked).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399