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Grp75 antibody [4A1]

Overexpression
Anti-Grp75 antibody [4A1] used in Western Blot (WB). GTX84330
Anti-Grp75 antibody [4A1] used in Western Blot (WB). GTX84330
Anti-Grp75 antibody [4A1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84330
Anti-Grp75 antibody [4A1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84330
Anti-Grp75 antibody [4A1] used in Western Blot (WB). GTX84330

Cat. No. GTX84330

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4A1

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($519)
See all Grp75 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:100
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human HSPA9 expression vector

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 9 , CRP40 , CSA , EVPLS , GRP-75 , GRP75 , HEL-S-124m , HSPA9B , MOT , MOT2 , MTHSP75 , PBP74 , SAAN , SIDBA4

Cellular Localization

Mitochondrion,Nucleolus

Background

Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.

Database

Research Area

DATA IMAGES

Anti-Grp75 antibody [4A1] used in Western Blot (WB). GTX84330

GTX84330 WB Image

WB analysis of various cell lines using GTX84330 Grp75 antibody [4A1].
Loading : 35 ug per lane

Anti-Grp75 antibody [4A1] used in Western Blot (WB). GTX84330

GTX84330 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84330 Grp75 antibody [4A1].
Loading : 10 ug per lane
Dilution : 1:500

Anti-Grp75 antibody [4A1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84330

GTX84330 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX84330 Grp75 antibody [4A1].

Anti-Grp75 antibody [4A1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84330

GTX84330 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Grp75 plasmid using GTX84330 Grp75 antibody [4A1].

Anti-Grp75 antibody [4A1] used in Western Blot (WB). GTX84330

GTX84330 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Grp75 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84330 Grp75 antibody [4A1].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519