Grp75 antibody detects Grp75 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Grp75 stained by Grp75 antibody (GTX104407) diluted at 1:500.
Blue: Hoechst 33342 staining.

Grp75 antibody detects Grp75 protein at mitochondria in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Grp75 antibody (GTX104407) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Grp75 antibody detects Grp75 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Grp75 stained by Grp75 antibody (GTX104407) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (6 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP75 antibody (GTX104407) diluted at 1:500.

Immunoprecipitation of GRP75 protein from HepG2 whole cell extracts using 5 μg of GRP75 antibody (GTX104407).
Western blot analysis was performed using GRP75 antibody (GTX104407) diluted at 1:2500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Grp75 antibody detects Grp75 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Grp75 stained by Grp75 antibody (GTX104407) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Grp75 antibody detects Grp75 protein at mitochondria in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Grp75 antibody (GTX104407) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Grp75 antibody detects Grp75 protein at mitochondria in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Grp75 antibody (GTX104407) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp75 antibody (GTX104407) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with Grp75 antibody (GTX104407) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Proteome Sci in 2014. PMID: 24405781