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Grp75 antibody

Anti-Grp75 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133283
Anti-Grp75 antibody used in IHC (Paraffin sections) (IHC-P). GTX133283
Anti-Grp75 antibody used in Western Blot (WB). GTX133283
Anti-Grp75 antibody used in IHC (Paraffin sections) (IHC-P). GTX133283
Anti-Grp75 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133283

Cat No. GTX133283

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse Grp75. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

heat shock protein 9,74kDa,Csa,Grp75,Hsc74,Hsp74,Hsp74a,Hspa9a,Mortalin,Mot-2,Mot2,Mthsp70,Pbp74

Cellular Localization

Mitochondrion

Background

Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.

Database

Research Area

DATA IMAGES
Anti-Grp75 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133283

GTX133283 ICC/IF Image

Grp75 antibody detects Grp75 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Grp75 protein stained by Grp75 antibody (GTX133283) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Grp75 antibody used in IHC (Paraffin sections) (IHC-P). GTX133283

GTX133283 IHC-P Image

Grp75 antibody detects Grp75 protein at cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Grp75 antibody (GTX133283) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp75 antibody used in Western Blot (WB). GTX133283

GTX133283 WB Image

Various tissue extracts (10 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp75 antibody (GTX133283) diluted at 1:2000.

Anti-Grp75 antibody used in IHC (Paraffin sections) (IHC-P). GTX133283

GTX133283 IHC-P Image

Grp75 antibody detects Grp75 protein at cytoplasm in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Grp75 antibody (GTX133283) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp75 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133283

GTX133283 IHC-Fr Image

Grp75 antibody detects Grp75 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Grp75 protein stained by Grp75 antibody (GTX133283) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169