APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 380-679 of human HSPA9 (NP_004125.3).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein family A (Hsp70) member 9 , CRP40 , CSA , EVPLS , GRP-75 , GRP75 , HEL-S-124m , HSPA9B , MOT , MOT2 , MTHSP75 , PBP74 , SAAN , SIDBA4
Cellular Localization
Mitochondrion,Nucleolus
Background
This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.[provided by RefSeq, May 2010]
Database
Research Area
DATA IMAGES
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GTX55649 IHC-P Image
IHC-P analysis of mouse heart tissue using GTX55649 Grp75 antibody.
Dilution : 1:100
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GTX55649 IHC-P Image
IHC-P analysis of human lung cancer tissue using GTX55649 Grp75 antibody.
Dilution : 1:100
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GTX55649 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX55649 Grp75 antibody.
Dilution : 1:100
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GTX55649 IHC-P Image
IHC-P analysis of human gastric cancer tissue using GTX55649 Grp75 antibody.
Dilution : 1:100
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GTX55649 WB Image
WB analysis of various sample lysates using GTX55649 Grp75 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX55649 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX55649 Grp75 antibody.
Blue : DAPI
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REFERENCE
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REVIEW
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