GeneTex
United States (US)

Grp78 antibody [C2C3], C-term

KO/KD
GTX102567_39680_20160728_WB_shRNA_watermark_19011506_807.jpg
GTX102567_39680_WB_19011506_837.jpg
GTX102567_39680_WB_M_19011506_473.jpg

Cat No. GTX102567

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

HepG2 , NIH-3T3 , JC , BCL-1

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Grp78. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Heat Shock Protein Family A (Hsp70) Member 5,Bip,Grp78,Hel-S-89N,Mif2,Hspa5

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Background

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX102567_39680_20160728_WB_shRNA_watermark_19011506_807.jpg

GTX102567 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [C2C3], C-term (GTX102567) diluted at 1:1000.

GTX102567_39680_WB_19011506_837.jpg

GTX102567 WB Image

Grp78 antibody detects Grp78 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Grp78 antibody (GTX102567) dilution: 1:1000

GTX102567_39680_WB_M_19011506_473.jpg

GTX102567 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX102567 diluted at 1:2000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169