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Grp78 antibody [HL1815]

Recombinant antibody KO/KD Orthogonal Overexpression
Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540
Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540
Anti-Grp78 antibody [HL1815] used in IHC (Paraffin sections) (IHC-P). GTX637540
Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540
Anti-Grp78 antibody [HL1815] used in IHC (Paraffin sections) (IHC-P). GTX637540
Anti-Grp78 antibody [HL1815] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637540
Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540

Cat. No. GTX637540

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1815

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Grp78 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Product Note

This antibody is specific for human HSPA5 protein, and it does not cross react with human HSPA1A, HSPA1L, HSPA2, HSPA6, HSPA8, and HSPA9 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Grp78

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HEL-S-89n , MIF2

Cellular Localization

Melanosome,Cytoplasm

Background

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]

Database

Research Area

DATA IMAGES

Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540

GTX637540 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [HL1815] (GTX637540) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540

GTX637540 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [HL1815] (GTX637540) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Grp78 antibody [HL1815] used in IHC (Paraffin sections) (IHC-P). GTX637540

GTX637540 IHC-P Image

Grp78 antibody [HL1815] detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Grp78 stained by Grp78 antibody [HL1815] (GTX637540) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540

GTX637540 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [HL1815] (GTX637540) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Grp78 antibody [HL1815] used in IHC (Paraffin sections) (IHC-P). GTX637540

GTX637540 IHC-P Image

Grp78 antibody [HL1815] detects Grp78 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Grp78 stained by Grp78 antibody [HL1815] (GTX637540) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp78 antibody [HL1815] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637540

GTX637540 ICC/IF Image

Grp78 antibody [HL1815] detects Grp78 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Grp78 stained by Grp78 antibody [HL1815] (GTX637540) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Grp78 antibody [HL1815] used in Western Blot (WB). GTX637540

GTX637540 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [HL1815] (GTX637540) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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Package List Price ($)
$ 399
$ 169
Custom Conjugations